ArticleThe Journal of cell biology1986
In vitro transport of a fluorescent nuclear protein and exclusion of non-nuclear proteins.
Article in The Journal of cell biology, 1986. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 81 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
81 citing papers in PubMed, 211 citations in OpenAlex.
- Measuring and Interpreting Nuclear Transport in Neurodegenerative Disease-The Example of C9orf72 ALS.International journal of molecular sciences · 2021Review
- Fluorescence-based quantification of nucleocytoplasmic transport.Methods (San Diego, Calif.) · 2019Article
- Cytoplasmic transport and nuclear import of plasmid DNA.Bioscience reports · 2017Review
- Small-molecule high-throughput screening utilizing Xenopus egg extract.Methods in molecular biology (Clifton, N.J.) · 2015Article
- Analysis of nuclear reconstitution, nuclear envelope assembly, and nuclear pore assembly using Xenopus in vitro assays.Methods in cell biology · 2014Article
- Screening for small molecule inhibitors of embryonic pathways: sometimes you gotta crack a few eggs.Bioorganic & medicinal chemistry · 2012Review
- Transmembrane protein-free membranes fuse into xenopus nuclear envelope and promote assembly of functional pores.The Journal of biological chemistry · 2009Article
- Transportin mediates nuclear entry of DNA in vertebrate systems.Traffic (Copenhagen, Denmark) · 2009Article
- FG/FxFG as well as GLFG repeats form a selective permeability barrier with self-healing properties.The EMBO journal · 2009Article
- Nucleocytoplasmic transport: a thermodynamic mechanism.HFSP journal · 2009Article
- The highly conserved nuclear lamin Ig-fold binds to PCNA: its role in DNA replication.The Journal of cell biology · 2008Article
- Cell-cycle-dependent phosphorylation of the nuclear pore Nup107-160 subcomplex.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Caspase-2-induced apoptosis requires bid cleavage: a physiological role for bid in heat shock-induced death.Molecular biology of the cell · 2006Article
- Nuclear localization signal peptides induce molecular delivery along microtubules.Biophysical journal · 2005Article
- The cytoplasmic filaments of the nuclear pore complex are dispensable for selective nuclear protein import.The Journal of cell biology · 2002Article
- Kinetics and mechanism of DNA uptake into the cell nucleus.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- S-Phase progression mediates activation of a silenced gene in synthetic nuclei.Molecular and cellular biology · 2000Article
- Roles of LAP2 proteins in nuclear assembly and DNA replication: truncated LAP2beta proteins alter lamina assembly, envelope formation, nuclear size, and DNA replication efficiency in Xenopus laevis extracts.The Journal of cell biology · 1999Article
- Chromosomal proteins HMG-14 and HMG-17 are released from mitotic chromosomes and imported into the nucleus by active transport.The Journal of cell biology · 1998Article
- Major binding sites for the nuclear import receptor are the internal nucleoporin Nup153 and the adjacent nuclear filament protein Tpr.The Journal of cell biology · 1998Article
21 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
An in vitro system was developed that provides a quick microscopic assay for nuclear transport. The assay uses an extract of Xenopus eggs, normal or synthetic nuclei, and a fluorescently labeled nuclear protein, nucleoplasmin. This in vitro system accurately mimics in vivo nuclear transport, both in exclusivity and in the amount of accumulation observed (up to 17-fold). Selective accumulation of fluorescent nucleoplasmin is observed microscopically within 30 min with rat liver nuclei, Xenopus embryonic nuclei, regrown Xenopus sperm nuclei, or nuclei reconstituted in vitro from bacteriophage lambda DNA. This transport requires the signal domain of nucleoplasmin. Furthermore, the ability of nuclei to accumulate nucleoplasmin directly correlates with their ability to exclude the fluorescent non-nuclear proteins, FITC-immunoglobulin and phycoerythrin. An active transport model would predict that nuclear transport be temperature- and energy-dependent and that inhibition of transport by either low temperature or energy depletion would be reversible. Both predictions were confirmed in our system. Nucleoplasmin accumulation increases with temperature, while the protein is completely excluded at 0 degrees C. The effects of low temperature are reversible. As found for 125I-labeled nucleoplasmin (Newmeyer, D. D., J. M. Lucocq, T. R. Bürglin, and E. M. De Robertis, 1986, EMBO (Eur. Mol. Biol. Organ.) J., 5:501-510), transport of fluorescent nucleoplasmin is inhibited by ATP depletion. This effect is reversed by later ATP addition. Under ATP-depleted conditions non-nuclear proteins continue to be excluded. These results argue for a direct role of ATP in transport rather than for a simple role in preserving envelope integrity. In a first step towards defining the minimum requirements for a transport medium, egg extracts were depleted of membrane vesicles. Membrane-depleted extracts neither support transport nor maintain the integrity of the nuclear envelope.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.