ArticlePLoS genetics2019
Precise staging of beetle horn formation in Trypoxylus dichotomus reveals the pleiotropic roles of doublesex depending on the spatiotemporal developmental contexts.
Article in PLoS genetics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 51 citations in OpenAlex.
- Rewiring of two conserved signalling hubs into one axis underlies convergent female-specific wing regression in insects.Nature ecology & evolution · 2026Article
- Sex-Specific doublesex Regulation Targeting the Color-Patterning Gene h Underlies the Evolution of Wing Sexual Dimorphism in the Harlequin Ladybug Harmonia axyridis.Evolution & development · 2026Article
- Introducing Exogenous DNA Vectors Directly intoBio-protocol · 2026Article
- Electroporation-mediated functional analysis method of genes in the giant insect Trypoxylus dichotomus.Scientific reports · 2025Article
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- Candidate target genes of the male-specific expressed Doublesex in the termite Reticulitermes speratus.PloS one · 2024Article
- Gene regulatory networks underlying the development and evolution of plasticity in horned beetles.Current opinion in insect science · 2023Review
- The draft genome sequence of the Japanese rhinoceros beetle Trypoxylus dichotomus septentrionalis towards an understanding of horn formation.Scientific reports · 2023Article
- Theme and variation in the evolution of insect sex determination.Journal of experimental zoology. Part B, Molecular and developmental evolution · 2023Review
- Evolutionary History of Sexual Differentiation Mechanism in Insects.Molecular biology and evolution · 2022Article
- RNA Interference Method for Gene Function Analysis in the Japanese Rhinoceros BeetleBio-protocol · 2022Article
- Noncanonical function of the Sex lethal gene controls the protogyny phenotype in Drosophila melanogaster.Scientific reports · 2022Article
- Novel Doublesex Duplication Associated with Sexually Dimorphic Development of Dogface Butterfly Wings.Molecular biology and evolution · 2021Article
- Evolution of sexual development and sexual dimorphism in insects.Current opinion in genetics & development · 2021Review
- Computational analyses decipher the primordial folding coding the 3D structure of the beetle horn.Scientific reports · 2021Article
- Phylogeny and biogeography of the Japanese rhinoceros beetle,Ecology and evolution · 2021Article
- Genetical control of 2D pattern and depth of the primordial furrow that prefigures 3D shape of the rhinoceros beetle horn.Scientific reports · 2020Article
- Variation in an Extreme Weapon: Horn Performance Differences across Rhinoceros Beetle (Insects · 2019Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many scarab beetles have sexually dimorphic exaggerated horns that are an evolutionary novelty. Since the shape, number, size, and location of horns are highly diverged within Scarabaeidae, beetle horns are an attractive model for studying the evolution of sexually dimorphic and novel traits. In beetles including the Japanese rhinoceros beetle Trypoxylus dichotomus, the sex differentiation gene doublesex (dsx) plays a crucial role in sexually dimorphic horn formation during larval-pupal development. However, knowledge of when and how dsx drives the gene regulatory network (GRN) for horn formation to form sexually dimorphic horns during development remains elusive. To address this issue, we identified a Trypoxylus-ortholog of the sex determination gene, transformer (tra), that regulates sex-specific splicing of the dsx pre-mRNA, and whose loss of function results in sex transformation. By knocking down tra function at multiple developmental timepoints during larval-pupal development, we estimated the onset when the sex-specific GRN for horn formation is driven. In addition, we also revealed that dsx regulates different aspects of morphogenetic activities during the prepupal and pupal developmental stages to form appropriate morphologies of pupal head and thoracic horn primordia as well as those of adult horns. Based on these findings, we discuss the evolutionary developmental background of sexually dimorphic trait growth in horned beetles.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.