ArticleJournal of neurophysiology2019
Autonomous patch-clamp robot for functional characterization of neurons in vivo: development and application to mouse visual cortex.
Article in Journal of neurophysiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- A robotic patch-clamp system with real-time localization and phase-synchronized capture of dynamic in vivo cells using micropipette resistance modelling.Microsystems & nanoengineering · 2026Article
- Plateau potentials are instructive signals for behavioral timescale synaptic plasticity in the neocortex.bioRxiv : the preprint server for biology · 2025Article
- Robotic Fast Patch Clamp in Brain Slices Based on Stepwise Micropipette Navigation and Gigaseal Formation Control.Sensors (Basel, Switzerland) · 2025Article
- Patch-walking, a coordinated multi-pipette patch clamp for efficiently finding synaptic connections.eLife · 2024Article
- Patch-walking: Coordinated multi-pipette patch clamp for efficiently finding synaptic connections.bioRxiv : the preprint server for biology · 2024Article
- High-throughput genetic manipulation of multicellular organisms using a machine-vision guided embryonic microinjection robot.Genetics · 2024Article
- Ensemble learning and ground-truth validation of synaptic connectivity inferred from spike trains.PLoS computational biology · 2024Article
- Immediate reuse of patch-clamp pipettes after ultrasonic cleaning.Scientific reports · 2024Article
- A biologically validated mathematical model for decoding epithelial apical, basolateral, and paracellular electrical properties.American journal of physiology. Cell physiology · 2023Article
- Neuron Contact Detection Based on Pipette Precise Positioning for Robotic Brain-Slice Patch Clamps.Sensors (Basel, Switzerland) · 2023Article
- Translational opportunities and challenges of invasive electrodes for neural interfaces.Nature biomedical engineering · 2023Review
- Smart imaging to empower brain-wide neuroscience at single-cell levels.Brain informatics · 2022Review
- Scaled, high fidelity electrophysiological, morphological, and transcriptomic cell characterization.eLife · 2021Article
- Automated microscope-independent fluorescence-guided micropipette.Biomedical optics express · 2021Article
- Measuring Absolute Membrane Potential Across Space and Time.Annual review of biophysics · 2021Review
- In Vivo Whole-Cell Patch-Clamp Methods: Recent Technical Progress and Future Perspectives.Sensors (Basel, Switzerland) · 2021Review
- Advances in 3D neuronal microphysiological systems: towards a functional nervous system on a chip.In vitro cellular & developmental biology. Animal · 2021Review
- Single neuron recording: progress towards high-throughput analysis.Bioelectronics in medicine · 2020Article
- High-yield, automated intracellular electrophysiology in retinal pigment epithelia.Journal of neuroscience methods · 2019Article
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Corrections and comments
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Authors and funding
11 authors.
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Abstract
Patch clamping is the gold standard measurement technique for cell-type characterization in vivo, but it has low throughput, is difficult to scale, and requires highly skilled operation. We developed an autonomous robot that can acquire multiple consecutive patch-clamp recordings in vivo. In practice, 40 pipettes loaded into a carousel are sequentially filled and inserted into the brain, localized to a cell, used for patch clamping, and disposed. Automated visual stimulation and electrophysiology software enables functional cell-type classification of whole cell-patched cells, as we show for 37 cells in the anesthetized mouse in visual cortex (V1) layer 5. We achieved 9% yield, with 5.3 min per attempt over hundreds of trials. The highly variable and low-yield nature of in vivo patch-clamp recordings will benefit from such a standardized, automated, quantitative approach, allowing development of optimal algorithms and enabling scaling required for large-scale studies and integration with complementary techniques.
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Registered trials
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