Evidence map›Paper›PMID 30968649›Full record

ArticleJournal of biomedical optics2019

Tunable structured illumination light sheet microscopy for background rejection and imaging depth in minimally processed tissues.

Joseph R Landry, Ryosuke Itoh, Jonathan M Li, Stephen S Hamann, Michael Mandella, Christopher H Contag, Olav Solgaard

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of biomedical optics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Joseph R LandryStanford University, Edward L. Ginzton Laboratory, Stanford, California, United States.
Ryosuke ItohSCREEN Holdings Co., Ltd., R&D Department, Kyoto, Japan.
Jonathan M LiStanford University, Edward L. Ginzton Laboratory, Stanford, California, United States.
Stephen S HamannStanford University, Edward L. Ginzton Laboratory, Stanford, California, United States.
Michael MandellaStanford University, Department of Radiology, Stanford, California, United States.
Christopher H ContagMichigan State University, Institute for Quantitative Health Science and Engineering, Department of, United States.
Olav SolgaardStanford University, Edward L. Ginzton Laboratory, Stanford, California, United States.

Funding

(PQC2)High-content Pathology with Confocal Microscope ArraysR01CA180152 · NCI · STANFORD UNIVERSITY · PI RIMM, DAVID L, SOLGAARD, OLAV · 2013 to 2016
$2.5M
NCI NIH HHS R01 CA180152
6 · The paper itself

Abstract

We demonstrate improved optical sectioning in light sheet fluorescence microscopy using tunable structured illumination (SI) frequencies to optimize image quality in scattering specimens. The SI patterns are generated coherently using a one-dimensional spatial light modulator for maximum pattern contrast, and the pattern spatial frequency is adjustable up to half the incoherent cutoff frequency of our detection objective. At this frequency, we demonstrate background reductions of 2 orders of magnitude.

Indexed as

Microscopy, FluorescenceAnimalsColonEquipment DesignImage Processing, Computer-AssistedLightMicePhantoms, ImagingScattering, Radiationimage reconstructionmicroscopyspatial light modulators

Identifiers

PMID30968649
PMCPMC6454294

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.