ArticleProceedings of the National Academy of Sciences of the United States of America2019
Myosin Va transport of liposomes in three-dimensional actin networks is modulated by actin filament density, position, and polarity.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 36 citations in OpenAlex.
- ARHGAP18 complexes with both YAP and Merlin and is required for basal actin bundles.Molecular biology of the cell · 2026Article
- The unconventional regulation of non-muscle myosins.Biophysical reviews · 2026Article
- Kinesin-1 autoinhibition tunes cargo transport by motor ensembles.Biophysical journal · 2025Article
- The Rho effector ARHGAP18 coordinates a Hippo pathway feedback loop through YAP and Merlin to regulate the cytoskeleton and epithelial cell polarity.bioRxiv : the preprint server for biology · 2025Article
- Reconstituted systems for studying the architecture and dynamics of actin networks.The Biochemical journal · 2025Review
- Multiple Mechanisms to Regulate Actin Functions: "Fundamental" Versus Lineage-Specific Mechanisms and Hierarchical Relationships.Biomolecules · 2025Review
- Kinesin-1-transported liposomes prefer to go straight in 3D microtubule intersections by a mechanism shared by other molecular motors.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Exploring the Role of the Plant Actin Cytoskeleton: From Signaling to Cellular Functions.International journal of molecular sciences · 2023Review
- Spatiotemporal three-dimensional transport dynamics of endocytic cargos and their physical regulations in cells.iScience · 2022Article
- Modeling myosin Va liposome transport through actin filament networks reveals a percolation threshold that modulates transport properties.Molecular biology of the cell · 2022Article
- Optimizing microtubule arrangements for rapid cargo capture.Biophysical journal · 2021Article
- Clinical and Neurobiological Aspects of TAO Kinase Family in Neurodevelopmental Disorders.Frontiers in molecular neuroscience · 2021Article
- Roles and regulation of myosin V interaction with cargo.Advances in biological regulation · 2021Review
- A novel labeling strategy reveals that myosin Va and myosin Vb bind the same dendritically polarized vesicle population.Traffic (Copenhagen, Denmark) · 2020Article
- F-actin disassembly factor MICAL1 binding to Myosin Va mediates cargo unloading during cytokinesis.Science advances · 2020Article
- Kymolyzer, a Semi-Autonomous Kymography Tool to Analyze Intracellular Motility.Current protocols in cell biology · 2020Article
- The role of the Arp2/3 complex in shaping the dynamics and structures of branched actomyosin networks.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Dynamic motions of molecular motors in the actin cytoskeleton.Cytoskeleton (Hoboken, N.J.) · 2019Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
The cell's dense 3D actin filament network presents numerous challenges to vesicular transport by teams of myosin Va (MyoVa) molecular motors. These teams must navigate their cargo through diverse actin structures ranging from Arp2/3-branched lamellipodial networks to the dense, unbranched cortical networks. To define how actin filament network organization affects MyoVa cargo transport, we created two different 3D actin networks in vitro. One network was comprised of randomly oriented, unbranched actin filaments; the other was comprised of Arp2/3-branched actin filaments, which effectively polarized the network by aligning the actin filament plus-ends. Within both networks, we defined each actin filament's 3D spatial position using superresolution stochastic optical reconstruction microscopy (STORM) and its polarity by observing the movement of single fluorescent reporter MyoVa. We then characterized the 3D trajectories of fluorescent, 350-nm fluid-like liposomes transported by MyoVa teams (∼10 motors) moving within each of the two networks. Compared with the unbranched network, we observed more liposomes with directed and fewer with stationary motion on the Arp2/3-branched network. This suggests that the modes of liposome transport by MyoVa motors are influenced by changes in the local actin filament polarity alignment within the network. This mechanism was supported by an in silico 3D model that provides a broader platform to understand how cellular regulation of the actin cytoskeletal architecture may fine tune MyoVa-based intracellular cargo transport.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.