ArticleBMC biology2019
Enabling cell-type-specific behavioral epigenetics in Drosophila: a modified high-yield INTACT method reveals the impact of social environment on the epigenetic landscape in dopaminergic neurons.
Article in BMC biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 30 citations in OpenAlex.
- Common Gene Expression Patterns in Environmental Model Organisms Exposed to Engineered Nanomaterials: A Meta-Analysis.Environmental science & technology · 2020Pooled it
- The Drosophila SWI/SNF complex subunit Bap60 is required for training-induced gene transcription during long-term memory formation.Genetics · 2026Article
- Cell-type-resolved RNA-seq reveals molecular signatures of sexual experience inNAR genomics and bioinformatics · 2026Article
- Review
- A memory transcriptome time course reveals essential long-term memory transcription factors.Nature communications · 2025Article
- Review
- Socialization causes long-lasting behavioral changes.Scientific reports · 2024Article
- The CoREST complex regulates multiple histone modifications temporal-specifically in clock neurons.Open biology · 2024Article
- Recovery from social isolation requires dopamine in males, but not the autism-related geneRoyal Society open science · 2024Article
- An integrative sensor of body states: how the mushroom body modulates behavior depending on physiological context.Learning & memory (Cold Spring Harbor, N.Y.) · 2024Review
- Article
- Review
- Deciphering Precise Gene Transcriptional Expression Using gwINTACT in Tomato.Frontiers in plant science · 2022Article
- In vivo tissue-specific chromatin profiling in Drosophila melanogaster using GFP-tagged nuclei.Genetics · 2021Article
- INT-Hi-C reveals distinct chromatin architecture in endosperm and leaf tissues of Arabidopsis.Nucleic acids research · 2021Article
- Transcriptome Analysis of NPFR Neurons Reveals a Connection Between Proteome Diversity and Social Behavior.Frontiers in behavioral neuroscience · 2021Article
- Meiocyte Isolation by INTACT and Meiotic Transcriptome Analysis inFrontiers in plant science · 2021Article
- Exposure to bisphenol A differentially impacts neurodevelopment and behavior in Drosophila melanogaster from distinct genetic backgrounds.Neurotoxicology · 2021Article
- Regulation of Social Stress and Neural Degeneration by Activity-Regulated Genes and Epigenetic Mechanisms in Dopaminergic Neurons.Molecular neurobiology · 2020Review
- The neuropeptide Drosulfakinin regulates social isolation-induced aggression inThe Journal of experimental biology · 2020Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
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Abstract
backgroundEpigenetic mechanisms play fundamental roles in brain function and behavior and stressors such as social isolation can alter animal behavior via epigenetic mechanisms. However, due to cellular heterogeneity, identifying cell-type-specific epigenetic changes in the brain is challenging. Here, we report the first use of a modified isolation of nuclei tagged in specific cell type (INTACT) method in behavioral epigenetics of Drosophila melanogaster, a method we call mini-INTACT.
resultsUsing ChIP-seq on mini-INTACT purified dopaminergic nuclei, we identified epigenetic signatures in socially isolated and socially enriched Drosophila males. Social experience altered the epigenetic landscape in clusters of genes involved in transcription and neural function. Some of these alterations could be predicted by expression changes of four transcription factors and the prevalence of their binding sites in several clusters. These transcription factors were previously identified as activity-regulated genes, and their knockdown in dopaminergic neurons reduced the effects of social experience on sleep.
conclusionsOur work enables the use of Drosophila as a model for cell-type-specific behavioral epigenetics and establishes that social environment shifts the epigenetic landscape in dopaminergic neurons. Four activity-related transcription factors are required in dopaminergic neurons for the effects of social environment on sleep.
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