ArticlePhilosophical transactions of the Royal Society of London. Series B, Biological sciences2019
Unveiling the function and regulation control of the DUF3129 family proteins in fungal infection of hosts.
Article in Philosophical transactions of the Royal Society of London. Series B, Biological sciences, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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22 citing papers in PubMed, 29 citations in OpenAlex.
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- CysDuF database: annotation and characterization of cysteine residues in domain of unknown function proteins based on cysteine post-translational modifications, their protein microenvironments, biochemical pathways, taxonomy, and diseases.Database : the journal of biological databases and curation · 2026Article
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- Glutamate dehydrogenase 2 is required for virulence by facilitating fungal growth in the host hemocoel.Virulence · 2025Article
- MaMsb2, a signaling mucin, is involved in conidiation, stress tolerances, and virulence in the entomopathogenic fungusVirulence · 2025Article
- Clinical Isolate ofOpen journal of medical microbiology · 2025Article
- Comparative transcriptome analysis to unveil genes affecting the host cuticle destruction in Metarhizium rileyi.Current genetics · 2023Article
- Transcription factors SltA and CrzA reversely regulate calcium homeostasis under calcium-limited conditions.Applied and environmental microbiology · 2023Article
- Key processes required for the different stages of fungal carnivory by a nematode-trapping fungus.PLoS biology · 2023Article
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- Host-Pathogen Interactions betweenJournal of fungi (Basel, Switzerland) · 2022Review
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- Conservative production of galactosaminogalactan in Metarhizium is responsible for appressorium mucilage production and topical infection of insect hosts.PLoS pathogens · 2021Article
- Identification of a key G-protein coupled receptor in mediating appressorium formation and fungal virulence against insects.Science China. Life sciences · 2021Article
- Transcription Factor CfSte12 of Colletotrichum fructicola Is a Key Regulator of Early Apple Glomerella Leaf Spot Pathogenesis.Applied and environmental microbiology · 2020Article
- A M35 family metalloprotease is required for fungal virulence against insects by inactivating host prophenoloxidases and beyond.Virulence · 2020Article
- The Transcription Factor SomA Synchronously Regulates Biofilm Formation and Cell Wall Homeostasis inmBio · 2020Article
- MrHex1 is Required for Woronin Body Formation, Fungal Development and Virulence inJournal of fungi (Basel, Switzerland) · 2020Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many prokaryotic and eukaryotic proteins contain domains of unknown function (DUFs). A DUF3129 family of proteins is widely encoded in the genomes of fungal pathogens. A few studies in plant and insect pathogens indicated that the DUF3129 genes are required for fungal penetration of host cuticles with an unclear mechanism. We found that a varied number of DUF3129 proteins is present in different fungal species and the proteins are evolutionarily diverged from each other at the inter- and intra-specific levels. By using the insect pathogenic fungus Metarhizium robertsii as a model, we performed experiments and found that the seven DUF3129 proteins encoded by this fungus are localized to cellular lipid droplets (LDs). Individual deletion of these genes did not affect fungal formation of the infection structure appressoria and the accumulation of LDs in fungal conidia. When compared with the wild-type (WT) strain, insect bioassays revealed that the virulence of most null mutants were significantly impaired during topical infection but not during injection of insects. Carbon starvation and the subsequent Western blot analysis indicated that the LD-specific perilipin protein was completely degraded in the WT cells whereas varied levels of perilipin could be detected in the mutant cells, which signified that depletion of LD content was delayed in mutant cells, and DUF3129 proteins are therefore involved in LD degradation. We also provided biochemical evidence that these DUF3129 genes are transcriptionally regulated by a yeast Ste12-like transcription factor. The findings of this study not only unveil the function of DUF3129 proteins but also better understand the diverse mechanism of fungus-host interactions. This article is part of the theme issue 'Biotic signalling sheds light on smart pest management'.
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