ArticlePolymers2018
KnowVolution of the Polymer-Binding Peptide LCI for Improved Polypropylene Binding.
Article in Polymers, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 67 citations in OpenAlex.
- MagNanoTrap Enrichment Empowers Ultra-Sensitive Quantification of Mixed Nanoplastic Particles From Environmental Water Samples.Angewandte Chemie (International ed. in English) · 2026Article
- LCI Engineering for Improved Polystyrene Binding: The Impact of Aromatic Amino Acid Substitutions.ACS omega · 2025Article
- Secretory Production of Heterologous Antimicrobial Peptides inEngineering in life sciences · 2025Article
- Review
- An enzymatic continuous-flow reactor based on a pore-size matching nano- and isoporous block copolymer membrane.Nature communications · 2024Article
- Directed Evolution of Material Binding Peptide for Polylactic Acid-specific Degradation in Mixed Plastic Wastes.ACS catalysis · 2023Article
- A Competitive High-Throughput Screening Platform for Designing Polylactic Acid-Specific Binding Peptides.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- A step forward to the optimized HlyA type 1 secretion system through directed evolution.Applied microbiology and biotechnology · 2023Article
- Gold nanoparticles-anchored peptides enable precise colorimetric estimation of microplastics.iScience · 2023Article
- Biomedical applications of solid-binding peptides and proteins.Materials today. Bio · 2023Review
- Optimized Hemolysin Type 1 Secretion System in Escherichia coli by Directed Evolution of the Hly Enhancer Fragment and Including a Terminator Region.Chembiochem : a European journal of chemical biology · 2022Article
- Sensitive and specific capture of polystyrene and polypropylene microplastics using engineered peptide biosensors.RSC advances · 2022Article
- Recombination of Single Beneficial Substitutions Obtained from Protein Engineering by Computer-Assisted Recombination (CompassR).Methods in molecular biology (Clifton, N.J.) · 2022Article
- Bioengineered textiles with peptide binders that capture SARS-CoV-2 viral particles.Communications materials · 2022Article
- Computer-Assisted Recombination (CompassR) Teaches us How to Recombine Beneficial Substitutions from Directed Evolution Campaigns.Chemistry (Weinheim an der Bergstrasse, Germany) · 2020Article
- Microbial and Enzymatic Degradation of Synthetic Plastics.Frontiers in microbiology · 2020Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The functionalization of polymer surfaces by polymer-binding peptides offers tremendous opportunities for directed immobilization of enzymes, bioactive peptides, and antigens. The application of polymer-binding peptides as adhesion promoters requires reliable and stable binding under process conditions. Molecular modes of interactions between material surfaces, peptides, and solvent are often not understood to an extent that enables (semi-) rational design of polymer-binding peptides, hindering the full exploitation of their potential. Knowledge-gaining directed evolution (KnowVolution) is an efficient protein engineering strategy that facilitates tailoring protein properties to application demands through a combination of directed evolution and computational guided protein design. A single round of KnowVolution was performed to gain molecular insights into liquid chromatography peak I peptide, 47 aa (LCI)-binding to polypropylene (PP) in the presence of the competing surfactant Triton X-100. KnowVolution yielded a total of 8 key positions (D19, S27, Y29, D31, G35, I40, E42, and D45), which govern PP-binding in the presence of Triton X-100. The recombination of two of the identified amino acid substitutions (Y29R and G35R; variant KR-2) yielded a 5.4 ± 0.5-fold stronger PP-binding peptide compared to LCI WT in the presence of Triton X-100 (1 mM). The LCI variant KR-2 shows a maximum binding capacity of 8.8 ± 0.1 pmol/cm² on PP in the presence of Triton X-100 (up to 1 mM). The KnowVolution approach enables the development of polymer-binding peptides, which efficiently coat and functionalize PP surfaces and withstand surfactant concentrations that are commonly used, such as in household detergents.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.