Evidence map›Paper›PMID 30965582›Full record

ArticleMolecules (Basel, Switzerland)2019

Enzymatic Synthesis of Trideuterated Sialosides.

Zhi-P Cai, Louis P Conway, Ying Y Huang, Wen J Wang, Pedro Laborda, Ting Wang, Ai M Lu, Hong L Yao, Kun Huang, Sabine L Flitsch and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecules (Basel, Switzerland), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.5field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 8 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 2 countries.

Zhi-P CaiGlycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. caizhipeng@njau.edu.cn.
Louis P ConwayGlycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. t2015030@njau.edu.cn.
Ying Y HuangGlycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. 2014108037@njau.edu.cn.
Wen J WangGlycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. 2014108004@njau.edu.cn.
Pedro LabordaSchool of Life Sciences, Nantong University, Nantong 226019, China. pedro.laborda@njau.edu.cn.
Ting WangGlycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. wangting@njau.edu.cn.
Ai M LuCollege of Sciences, Nanjing Agricultural University, Nanjing 210095, China. luaimin@njau.edu.cn.
Hong L YaoGlycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. 2013208011@njau.edu.cn.
Kun HuangManchester Institute of Biotechnology, University of Manchester, Manchester M1 7DN, UK. kun.huang-3@manchester.ac.uk.
Sabine L FlitschManchester Institute of Biotechnology, University of Manchester, Manchester M1 7DN, UK. sabine.flitsch@manchester.ac.uk.
Li LiuGlycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. lichen.liu@njau.edu.cn.
Josef VoglmeirGlycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. josef.voglmeir@njau.edu.cn.ORCID 0000-0002-4096-4926
Nanjing Agricultural University · CNUniversity of Manchester · GBNantong University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Sialic acids are a family of acidic monosaccharides often found on the termini of cell surface proteins or lipid glycoconjugates of higher animals. Herein we describe the enzymatic synthesis of the two isotopically labeled sialic acid derivatives d₃-X-Gal-α-2,3-Neu5Ac and d₃-X-Gal-α-2,3-Neu5Gc. Using deuterium oxide as the reaction solvent, deuterium atoms could be successfully introduced during the enzymatic epimerization and aldol addition reactions when the sialosides were generated. NMR and mass spectrometric analyses confirmed that the resulting sialosides were indeed tri-deuterated. These compounds may be of interest as internal standards in liquid chromatography/mass spectrometric assays for biochemical or clinical studies of sialic acids. This was further exemplified by the use of this tri-deuterated sialosides as internal standards for the quantification of sialic acids in meat and egg samples.

Indexed as

Deuterium OxideEnzymesMagnetic Resonance SpectroscopyMass SpectrometryOxo-Acid-LyasesRacemases and EpimerasesSialic AcidsDeuterium OxideEnzymesN-acetylneuraminate lyaseN-acetylneuraminate synthaseOxo-Acid-LyasesRacemases and EpimerasesSialic Acidsisotope labeled carbohydratesN-glycolylneuraminic acidsialic acid biosynthesissialic acid quantification

Identifiers

PMID30965582
PMCPMC6479850
OpenAlexW3001492033

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.