ArticleCytometry. Part A : the journal of the International Society for Analytical Cytology2019
Titrating Complex Mass Cytometry Panels.
Article in Cytometry. Part A : the journal of the International Society for Analytical Cytology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Bemcentinib as monotherapy and in combination with low-dose cytarabine in acute myeloid leukemia patients unfit for intensive chemotherapy: a phase 1b/2a trial.Nature communications · 2025Trial
- Protocol for live cell barcoding and immunophenotyping of human hematological malignancies using cytometry by time of flight.STAR protocols · 2026Article
- Article
- Regulatory T-Cell Phenotyping Using CyTOF.Methods in molecular biology (Clifton, N.J.) · 2023Article
- CyTOFFrontiers in immunology · 2022Review
- Multiplexed single-cell analysis of organoid signaling networks.Nature protocols · 2021Review
- Mass cytometry profiling of human dendritic cells in blood and tissues.Nature protocols · 2021Review
- Article
- Mass Cytometry Defines Virus-Specific CD4ImmunoHorizons · 2020Article
- Article
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Authors and funding
9 authors.
Funding
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Abstract
We describe here a simple and efficient antibody titration approach for cell-surface markers and intracellular cell signaling targets for mass cytometry. The iterative approach builds upon a well-characterized backbone panel of antibodies and analysis using bioinformatic tools such as SPADE. Healthy peripheral blood and bone marrow cells are stained with a pre-optimized "backbone" antibody panel in addition to the progressively diluted (titrated) antibodies. Clustering based on the backbone panel enables the titration of each antibody against a rich hematopoietic background and assures that nonspecific binding and signal spillover can be quantified accurately. Using a slightly expanded backbone panel, antibodies quantifying changes in transcription factors and phosphorylated antigens are titrated on ex vivo stimulated cells to optimize sensitivity and evaluate baseline expression. Based on this information, complex panels of antibodies can be thoroughly optimized for use on healthy whole blood and bone marrow and are easily adaptable to the investigation of samples from for example clinical studies. © 2019 The Authors. Cytometry Part A published by Wiley Periodicals, Inc. on behalf of International Society for Advancement of Cytometry.
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