Evidence map›Paper›PMID 30963568›Full record

ArticleInternational journal of cancer2019

Long noncoding RNA HOTAIR promotes invasion of breast cancer cells through chondroitin sulfotransferase CHST15.

Liang-Chih Liu, Yuan-Liang Wang, Pei-Le Lin, Xiang Zhang, Wei-Chung Cheng, Shu-Hsuan Liu, Chih-Jung Chen, Yu Hung, Chia-Ing Jan, Ling-Chu Chang and 3 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of cancer, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
33citing papers in PubMed, 1 pooled it
2.5field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

33 citing papers in PubMed, 1 synthesis or guideline pooled it, 49 citations in OpenAlex.

  1. Pooled it
  2. Review
  3. Review
  4. Article
  5. Article
  6. Review
  7. Article
  8. Review
  9. Review
  10. Review
  11. Article
  12. Article
  13. Article
  14. Article
  15. Review
  16. Article
  17. Article
  18. Article
  19. Review
  20. Aptamer-guided targeting of the intracellular long-noncoding RNAAmerican journal of cancer research · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 5 institutions in 2 countries.

Liang-Chih LiuDepartment of Medicine, College of Medicine, China Medical University, Taichung, Taiwan.
Yuan-Liang WangGraduate Institute of Biomedical Sciences, College of Medicine, China Medical University, Taichung, Taiwan.
Pei-Le LinCenter for Molecular Medicine, China Medical University Hospital, Taichung, Taiwan.
Xiang ZhangDepartment of Environmental Health, University of Cincinnati, Cincinnati, OH.
Wei-Chung ChengGraduate Institute of Biomedical Sciences, College of Medicine, China Medical University, Taichung, Taiwan.
Shu-Hsuan LiuGraduate Institute of Biomedical Sciences, College of Medicine, China Medical University, Taichung, Taiwan.
Chih-Jung ChenDepartment of Surgery, China Medical University Hospital, Taichung, Taiwan.
Yu HungGraduate Institute of Biomedical Sciences, College of Medicine, China Medical University, Taichung, Taiwan.
Chia-Ing JanDivision of Molecular Pathology, Department of Pathology, China Medical University Hospital, Taichung, Taiwan.
Ling-Chu ChangChinese Medicinal Research and Development Center, China Medical University Hospital, Taichung, Taiwan.
Xiaoyang QiDepartment of Hematology Oncology, University of Cincinnati, Cincinnati, OH.
Linda C Hsieh-WilsonDivision of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA.
Shao-Chun WangGraduate Institute of Biomedical Sciences, College of Medicine, China Medical University, Taichung, Taiwan.ORCID 0000-0002-5477-1682
China Medical University · TWAsia University · TWCalifornia Institute of Technology · USCincinnati Health Department · USOncology Hematology Care · US

Funding

A chemical approach to elucidating the structure-function relationships of chondronitin sulfate glycosaminoglycansR01GM093627 · NIGMS · CALIFORNIA INSTITUTE OF TECHNOLOGY · PI HSIEH-WILSON, LINDA C · 2010 to 2018
$3.6M
NIGMS NIH HHS R01 GM093627
6 · The paper itself

Abstract

The long noncoding RNA HOTAIR plays significant roles in promoting cancer metastasis. However, how it conveys an invasive advantage in cancer cells is not clear. Here we identify the chondroitin sulfotransferase CHST15 (GalNAc4S-6ST) as a novel HOX transcript antisense intergenic RNA (HOTAIR) target gene using RNA profiling and show that CHST15 is required for HOTAIR-mediated invasiveness in breast cancer cells. CHST15 catalyzes sulfation of the C6 hydroxyl group of the N-acetyl galactosamine 4-sulfate moiety in chondroitin sulfate to form the 4,6-disulfated chondroitin sulfate variant known as the CS-E isoform. We show that HOTAIR is necessary and sufficient for CHST15 transcript expression. Inhibition of CHST15 by RNA interference abolished cell invasion promoted by HOTAIR but not on HOTAIR-mediated migratory activity. Conversely, reconstitution of CHST15 expression rescued the invasive activity of HOTAIR-depleted cells. In corroboration with this mechanism, blocking cell surface chondroitin sulfate using a pan-CS antibody or an antibody specifically recognizes the CS-E isoform significantly suppressed HOTAIR-induced invasion. Inhibition of CHST15 compromised tumorigenesis and metastasis in orthotopic breast cancer xenograft models. Furthermore, the expression of HOTAIR closely correlated with the level of CHST15 protein in primary as well as metastatic tumor lesions. Our results demonstrate a novel mechanism underlying the function of HOTAIR in tumor progression through programming the context of cell surface glycosaminoglycans. Our results further establish that the invasive and migratory activities downstream of HOTAIR are distinctly regulated, whereby CHST15 preferentially controls the arm of invasiveness. Thus, the HOTAIR-CHST15 axis may provide a new avenue toward novel therapeutic strategies and prognosis biomarkers for advanced breast cancer.

Indexed as

Breast NeoplasmsCell Line, TumorCell MovementCell ProliferationDisease ProgressionFemaleGene Expression Regulation, NeoplasticHumansMembrane GlycoproteinsNeoplasm InvasivenessRNA InterferenceRNA, Long NoncodingRNA, Small InterferingSulfotransferaseschondroitin 4-sulfotransferaseCHST15 protein, humanHOTAIR long untranslated RNA, humanMembrane GlycoproteinsRNA, Long NoncodingRNA, Small InterferingSulfotransferasesbreast cancerchondroitin sulfateCHST15glycosaminoglycansHOTAIRinvasionlong noncoding RNARNA-seq

Identifiers

PMID30963568
PMCPMC7263025
OpenAlexW2939038464

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.