ArticleMolecular biotechnology2019
High Conversion of D-Fructose into D-Allulose by Enzymes Coupling with an ATP Regeneration System.
Article in Molecular biotechnology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 31 citations in OpenAlex.
- Optimization of fermentation conditions for whole cell catalytic synthesis of D-allulose by engineering Escherichia coli.Scientific reports · 2024Article
- Enhancing L-asparagine Production Through In Vivo ATP Regeneration System Utilizing Glucose Metabolism of Escherichia coli.Applied biochemistry and biotechnology · 2024Article
- Enhancing L-Asparagine Bioproduction Efficiency Through L-Asparagine Synthetase and Polyphosphate Kinase-Coupled Conversion and ATP Regeneration.Applied biochemistry and biotechnology · 2024Article
- Awakening the natural capability of psicose production in Escherichia coli.NPJ science of food · 2023Article
- Highly Efficient Synthesis of Rare Sugars from Glycerol in Endotoxin-FreeFoods (Basel, Switzerland) · 2023Article
- A high-throughput dual system to screen polyphosphate kinase mutants for efficient ATP regeneration in L-theanine biocatalysis.Biotechnology for biofuels and bioproducts · 2023Article
- Metabolically EngineeredFrontiers in bioengineering and biotechnology · 2022Article
- Research Advances of d-allulose: An Overview of Physiological Functions, Enzymatic Biotransformation Technologies, and Production Processes.Foods (Basel, Switzerland) · 2021Review
- D-Allulose 3-epimerase of Bacillus sp. origin manifests profuse heat-stability and noteworthy potential of D-fructose epimerization.Microbial cell factories · 2021Article
- Synthesis of L-asparagine Catalyzed by a Novel Asparagine Synthase Coupled With an ATP Regeneration System.Frontiers in bioengineering and biotechnology · 2021Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
D-Allulose is a rare monosaccharide that exists in extremely small quantities in nature, and it is also hard to prepare at a large scale via chemical or enzyme synthetic route due to low conversion and downstream separation complexity. Using D-psicose epimerase and L-rhamnulose kinase, a method enabling high conversion of D-allulose from D-fructose without the need for a tedious isomer separation step was established recently. However, this method requires expensive ATP to facilitate the reaction. In the present study, an ATP regenerate system was developed coupling with polyphosphate kinase. In our optimized reaction with purified enzymes, the conversion rate of 99% D-fructose was achieved at the concentrations of 2 mM ATP, 5 mM polyphosphate, 20 mM D-fructose, and 20 mM Mg
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Registered trials
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