Evidence map›Paper›PMID 30963405›Full record

ArticleInfection2019

Analysis of hepatitis B virus-mixed genotype infection by ultra deep pyrosequencing in Sudanese patients, 2015-2016.

Khalid Abdallah Enan, Claudia Minosse, Abdel Rahim Mohammed El Hussein, Marina Selleri, Emanuela Giombini, Maria Rosaria Capobianchi, Isam Mohamed Elkhidir, Mohamed Omer Mustafa, Osama Mohamed Khair, Dina Ahamed Hassan and 1 more

Abstract read
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In one paragraph

Article in Infection, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Khalid Abdallah Enan *Department of Virology, Central Laboratory, The Ministry of Higher Education and Scientific Research, 7099, Khartoum, Sudan.
Claudia Minosse *Laboratory of Virology, National Institute for Infectious Diseases "Lazzaro Spallanzani"-IRCCS, Via Portuense, 292, 00149, Rome, Italy.
Abdel Rahim Mohammed El HusseinDepartment of Virology, Central Laboratory, The Ministry of Higher Education and Scientific Research, 7099, Khartoum, Sudan.
Marina SelleriLaboratory of Virology, National Institute for Infectious Diseases "Lazzaro Spallanzani"-IRCCS, Via Portuense, 292, 00149, Rome, Italy.
Emanuela GiombiniLaboratory of Virology, National Institute for Infectious Diseases "Lazzaro Spallanzani"-IRCCS, Via Portuense, 292, 00149, Rome, Italy.
Maria Rosaria CapobianchiLaboratory of Virology, National Institute for Infectious Diseases "Lazzaro Spallanzani"-IRCCS, Via Portuense, 292, 00149, Rome, Italy.
Isam Mohamed ElkhidirDepartment of Microbiology and Parasitology, Faculty of Medicine, University of Khartoum, 7099, Khartoum, Sudan.
Mohamed Omer MustafaDepartment of Virology, Central Laboratory, The Ministry of Higher Education and Scientific Research, 7099, Khartoum, Sudan.
Osama Mohamed KhairDepartment of Microbiology and Parasitology, Faculty of Medicine, University of Khartoum, 7099, Khartoum, Sudan.
Dina Ahamed HassanDepartment of Virology, Central Laboratory, The Ministry of Higher Education and Scientific Research, 7099, Khartoum, Sudan.
Anna Rosa GarbugliaLaboratory of Virology, National Institute for Infectious Diseases "Lazzaro Spallanzani"-IRCCS, Via Portuense, 292, 00149, Rome, Italy. argarbuglia@iol.it.

Funding

Ministero della Salute linea 3 hepatitisMinistero della Salute ricerca corrente
6 · The paper itself

Abstract

purposeThe frequency of detection of HBV co-infection with multiple HBV genotypes is influenced by the detection method; usually co-infections are detected by multiplex PCR or hybridization assays, and are rarely confirmed by sequencing and conventional cloning. The objective of this study was to confirm by ultra-deep pyrosequencing (UDPS) mixed HBV infections, previously detected by multiplex-nested PCR.

methodsSixteen samples from HBV co-infected Sudanese patients detected by multiplex-nested PCR, were amplified targeting the P/S region and sequenced by UDPS.

resultsThe only genotypes identified using UDPS were D and E, while A, B, C and F genotypes, previously detected by multiplex-nested PCR, were not detected. Specifically, 10 samples were shown to be mono-infected (D or E); in 3 out of 10 mono-infected D patients, a subtype combination was observed: D1 + D7 in 2 cases and D2 + D6 in 1 case. The remaining 6 subjects were D + E co-infected (harboring different mixtures of D subtypes).

conclusionsOverall, UDPS is more effective than multiplex-nested PCR for identifying multiple HBV genotypes and subtypes infections.

Indexed as

High-Throughput Nucleotide SequencingAmino Acid SequenceCoinfectionDNA, ViralGenotypeHepatitis BHepatitis B virusHumansMutationPhylogenySequence Analysis, DNASudanDNA, ViralCo-infectionHepatitis B virusHigh-throughput nucleotide sequencingSudan

Identifiers

PMID30963405

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.