ArticleAnnals of biomedical engineering2020
Independent, Controllable Stretch-Perfusion Bioreactor Chambers to Functionalize Cell-Seeded Decellularized Tendons.
Article in Annals of biomedical engineering, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 26 citations in OpenAlex.
- Decellularized extracellular matrix biomaterials for regenerative therapies: Advances, challenges and clinical prospects.Bioactive materials · 2024Review
- Equine tendon mechanical behaviour: Prospects for repair and regeneration applications.Veterinary medicine and science · 2023Review
- Tendon Extracellular Matrix Promotes Myotendinous Junction Protein Expression in Engineered Muscle Tissue under Both Static and Mechanically Stimulated Culture Conditions.Journal of tissue engineering and regenerative medicine · 2023Article
- Impact of Electrospun Piezoelectric Core-Shell PVDFhfp/PDMS Mesh on Tenogenic and Inflammatory Gene Expression in Human Adipose-Derived Stem Cells: Comparison of Static Cultivation with Uniaxial Cyclic Tensile Stretching.Bioengineering (Basel, Switzerland) · 2022Article
- Advanced Robotics to Address the Translational Gap in Tendon Engineering.Cyborg and bionic systems (Washington, D.C.) · 2022Review
- Bioengineering lungs: An overview of current methods, requirements, and challenges for constructing scaffolds.Frontiers in bioengineering and biotechnology · 2022Review
- Autologous bone marrow-derived mesenchymal stem cells provide complete regeneration in a rabbit model of the Achilles tendon bundle rupture.International orthopaedics · 2021Article
- Sterilization and disinfection methods for decellularized matrix materials: Review, consideration and proposal.Bioactive materials · 2021Review
- The Lack of a Representative Tendinopathy Model Hampers Fundamental Mesenchymal Stem Cell Research.Frontiers in cell and developmental biology · 2021Review
- Achilles Tendon Repair by Decellularized and Engineered Xenografts in a Rabbit Model.Stem cells international · 2019Article
- A universal multi-platform 3D printed bioreactor chamber for tendon tissue engineering.Journal of tissue engineeringArticle
- Decellularised scaffolds: just a framework? Current knowledge and future directions.Journal of tissue engineeringReview
- Multimodal analysis of the differential effects of cyclic strain on collagen isoform composition, fibril architecture and biomechanics of tissue engineered tendon.Journal of tissue engineeringArticle
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
Tissue-engineered decellularized matrices can progress clinical replacement of full-thickness ruptures or tendon defects. This study develops and validates a custom-made automated bioreactor, called oscillating stretch-perfusion bioreactor (OSPB), consisting of multiple, independent culture chambers able to combine a bidirectional perfusion with a programmable, uniaxial strain to functionalize cell-seeded decellularized tendons. Decellularized tendon matrices were seeded on their surfaces and within the tendon fibers with mesenchymal stem cells. Then, they were subjected to a bidirectional perfusion and programmed stretching cycles of 15-30-60 min on-off two times per day for 7 days of culture. In vitro analyses showed viable cells, homogenously distributed on the surface of the constructs. More importantly, cell-seeded decellularized tendon grafts undergoing cyclic load in our bioreactor had a superior production and organization of newly formed collagen matrix compared to static cultured constructs. The coherency and local alignment of the new collagen deposition within the inner injected channels quantitatively supported histological findings. The designed OSPB could be considered a unique, cost-effective system able to involve multiple independently controlled chambers in terms of biological and mechanical protocols. This system allows parallel processing of several customized tendon constructs to be used as grafts to enhance the surgical repair of large tendon defects.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.