Evidence map›Paper›PMID 30962821›Full record

ArticleBiotechnology for biofuels2019

CRISPR-Cas ribonucleoprotein mediated homology-directed repair for efficient targeted genome editing in microalgae

Mihris Ibnu Saleem Naduthodi, Prarthana Mohanraju, Christian Südfeld, Sarah D'Adamo, Maria J Barbosa, John van der Oost

Open access · goldAbstract read
In one paragraph

Article in Biotechnology for biofuels, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.

0numbers the graph read from it
0cells of the map it votes in
33citing papers in PubMed
3.9field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

33 citing papers in PubMed, 112 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
  4. An acetyltransferase family required for biosynthesis of diverse marine carotenoids.Proceedings of the National Academy of Sciences of the United States of America · 2026
    Article
  5. Article
  6. Harnessing microalgae for bioproducts: innovations in synthetic biology.World journal of microbiology & biotechnology · 2025
    Review
  7. Article
  8. Review
  9. Review
  10. Article
  11. Progress and challenges in CRISPR/Cas applications in microalgae.Journal of microbiology (Seoul, Korea) · 2025
    Review
  12. Review
  13. Review
  14. Article
  15. Review
  16. Marine drugs · 2024
    Review
  17. Review
  18. Review
  19. Modulation of xanthophyll cycle impacts biomass productivity in the marine microalgaProceedings of the National Academy of Sciences of the United States of America · 2023
    Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Mihris Ibnu Saleem Naduthodi1Laboratory of Microbiology, Wageningen University, Stippeneng 4, 6708 PD Wageningen, The Netherlands.
Prarthana Mohanraju1Laboratory of Microbiology, Wageningen University, Stippeneng 4, 6708 PD Wageningen, The Netherlands.
Christian Südfeld2Bioprocess Engineering Department, Wageningen University, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands.
Sarah D'Adamo2Bioprocess Engineering Department, Wageningen University, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands.
Maria J Barbosa2Bioprocess Engineering Department, Wageningen University, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands.
John van der Oost1Laboratory of Microbiology, Wageningen University, Stippeneng 4, 6708 PD Wageningen, The Netherlands.ORCID 0000-0001-5024-1871
Wageningen University & Research · NL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundMicroalgae are considered as a sustainable feedstock for the production of biofuels and other value-added compounds. In particular,

resultsIn this study, we report for the first time an alternative approach for CRISPR-Cas-mediated genome editing in

conclusionWe show that the delivery of Cas RNP by electroporation can be an alternative approach to the presently reported plasmid-based Cas9 method for generating mutants of

Indexed as

Cas12aCas9CRISPRGenome editingHomologous recombinationHomology-directed repairMicroalgaeNannochloropsisRibonucleoproteins

Identifiers

PMID30962821
PMCPMC6432748
OpenAlexW2923103077

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.