ArticleNature protocols2019
Regeneration of a bioengineered 3D integumentary organ system from iPS cells.
Article in Nature protocols, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Bioengineering strategies for regeneration of skin integrity: A literature review.Regenerative therapy · 2025Review
- Organoids/organs-on-chips towards biomimetic human artificial skin.Burns & trauma · 2025Review
- Materials-based hair follicle engineering: Basic components and recent advances.Materials today. Bio · 2024Review
- Innovative Strategies for Hair Regrowth and Skin Visualization.Pharmaceutics · 2023Review
- iPSC-based approach for human hair follicle regeneration.Frontiers in cell and developmental biology · 2023Review
- Cancer spheroids derived exosomes reveal more molecular features relevant to progressed cancer.Biochemistry and biophysics reports · 2021Article
- Generation of Skin Organoids: Potential Opportunities and Challenges.Frontiers in cell and developmental biology · 2021Review
- RNA sequence analysis of dermal papilla cells' regeneration in 3D culture.Journal of cellular and molecular medicine · 2020Article
- Toward Combined Cell and Gene Therapy for Genodermatoses.Cold Spring Harbor perspectives in biology · 2020Review
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Organ systems play essential roles in the physiological functions required for homeostasis. A 3D integumentary organ system (3D-IOS) comprises the skin and skin appendages such as hair follicles and sebaceous glands. This protocol describes how to induce the differentiation of murine induced pluripotent stem (iPS) cells into a 3D-IOS. First, iPS cells are grown for 7 d under conditions that encourage the formation of embryoid bodies (EBs). The iPS cell-derived EBs are stimulated by Wnt10b one day before transplantation of multiple EBs in vivo (a method we describe as the clustering-dependent embryoid body (CDB) transplantation method). After a further 30 d, the transplanted EBs will have differentiated into a 3D-IOS containing mature hair follicles and sebaceous glands. These can be removed and transplanted into wounds in the skin of other mice. After transplantation of a 3D-IOS, the organ system shows full physiological function in vivo starting 14 d following transplant. Thus, this protocol enables a whole functional organ system to be generated from pluripotent stem cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.