Evidence map›Paper›PMID 30962569›Full record

ArticleNature microbiology2019

Functional genomics of the rapidly replicating bacterium Vibrio natriegens by CRISPRi.

Henry H Lee, Nili Ostrov, Brandon G Wong, Michaela A Gold, Ahmad S Khalil, George M Church

Open access · greenAbstract read
PubMed Publisher
In one paragraph

Article in Nature microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 111 papers.

0numbers the graph read from it
0cells of the map it votes in
111citing papers in PubMed
21.7field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

111 citing papers in PubMed, 228 citations in OpenAlex.

  1. Metabolic engineering ofSynthetic and systems biotechnology · 2026
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  2. Formate-driven efficient pyruvate biosynthesis inSynthetic and systems biotechnology · 2026
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  6. Heterologous expression ofEngineering microbiology · 2026
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  13. Host-Associated Biofilms:Microorganisms · 2025
    Review
  14. Article
  15. Distantly related bacteria share a rigid proteome allocation strategy with flexible enzyme kinetics.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  16. Article
  17. Article
  18. Article
  19. Article
  20. Article

51 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Henry H Lee *Department of Genetics, Harvard Medical School, Boston, MA, USA. hhlee@genetics.med.harvard.edu.ORCID http://orcid.org/0000-0002-0577-2275
Nili Ostrov *Department of Genetics, Harvard Medical School, Boston, MA, USA.ORCID http://orcid.org/0000-0002-2157-587X
Brandon G WongDepartment of Biomedical Engineering and Biological Design Center, Boston University, Boston, MA, USA.ORCID http://orcid.org/0000-0003-1639-7594
Michaela A GoldDepartment of Genetics, Harvard Medical School, Boston, MA, USA.
Ahmad S KhalilDepartment of Biomedical Engineering and Biological Design Center, Boston University, Boston, MA, USA.ORCID http://orcid.org/0000-0002-8214-0546
George M ChurchDepartment of Genetics, Harvard Medical School, Boston, MA, USA. gchurch@genetics.med.harvard.edu.ORCID http://orcid.org/0000-0003-3535-2076
Harvard University · USBoston University · US

Funding

Combatting antibiotic resistance with synthetic biology technologiesDP2AI131083 · NIAID · BOSTON UNIVERSITY (CHARLES RIVER CAMPUS) · PI KHALIL, AHMAD SAMIR · 2016 to 2016
$2.5M
6 · The paper itself

Abstract

The fast-growing Gram-negative bacterium Vibrio natriegens is an attractive microbial system for molecular biology and biotechnology due to its remarkably short generation time

Indexed as

CRISPR-Cas SystemsBacterial ProteinsChromosomes, BacterialClustered Regularly Interspaced Short Palindromic RepeatsCulture MediaGene Knockdown TechniquesGene LibraryGenome, BacterialGenomicsVibrioBacterial ProteinsCulture Media

Identifiers

PMID30962569
OpenAlexW2929007946

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.