Evidence map›Paper›PMID 30962534›Full record

ArticleGene therapy2019

Regulated ex vivo regional gene therapy for bone repair using an inducible caspase-9 suicide gene system.

Sofia Bougioukli, Venus Vakhshori, Brandon Ortega, Osamu Sugiyama, Jay Lieberman

Abstract read
PubMed Publisher
In one paragraph

Article in Gene therapy, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.5field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 6 citations in OpenAlex.

  1. Review
  2. Gene therapy for bone healing: lessons learned and new approaches.Translational research : the journal of laboratory and clinical medicine · 2021
    Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Sofia BougioukliDepartment of Orthopaedic Surgery, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.
Venus VakhshoriDepartment of Orthopaedic Surgery, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.
Brandon OrtegaDepartment of Orthopaedic Surgery, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.
Osamu SugiyamaDepartment of Orthopaedic Surgery, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.
Jay LiebermanDepartment of Orthopaedic Surgery, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA. Jay.Lieberman@med.usc.edu.
University of Southern California · US

Funding

The Use of Regional Gene Delivery to Heal Critical Sized Bone DefectsR01AR057076 · NIAMS · UNIVERSITY OF SOUTHERN CALIFORNIA · PI LIEBERMAN, JAY R. · 2010 to 2021
$3.9M
U.S. Department of Health & Human Services | NIH | National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS) R01AR057076
6 · The paper itself

Abstract

In order to adapt ex vivo regional gene therapy for clinical applications in orthopaedic surgery, safety issues must be considered. In this study we developed a suicide approach using a dual gene expression two step transcriptional amplification lentiviral vector (LV-TSTA) encoding BMP-2 and an inducible caspase 9 (iC9) system that selectively induces apoptosis upon activation with a chemical inducer of dimerization (CID). Transduction of rat bone marrow stromal cells (RBMSCs) with LV-TSTA-iC9/BMP-2 led to abundant BMP-2 production (90.3 ± 7.9 ng/24 h/10

Indexed as

ApoptosisBone RegenerationAnimalsBone Morphogenetic Protein 2Caspase 6Cells, CulturedFemaleGenetic TherapyGenetic VectorsLentivirusMiceMice, Inbred NODProtein MultimerizationRatsBone Morphogenetic Protein 2Caspase 6

Identifiers

PMID30962534
OpenAlexW2932011040

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.