ArticleJournal of the Royal Society, Interface2019
In vitro cell migration quantification method for scratch assays.
Article in Journal of the Royal Society, Interface, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT06605365 (Ophthalmic Solution in Drye Eye Patients), which is not on this map. Cited by 79 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Ophthalmic Solution in Drye Eye Patients
Who cites it
79 citing papers in PubMed.
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- The In Vitro Wound-Scratch Assay: Applications, Technical Advances, and Limitations in Wound Healing Research.International wound journal · 2026Review
- Optimized Wound Healing Assay to Study Extracellular Vesicle-Driven Glioblastoma Cell Migration.Methods and protocols · 2026Article
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- Enhancing Wound Healing with a Novel Alginate/Eggshell Membrane-Based Hydrogel Enriched with Red Ginseng Roots and Centella Asiatica.Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI) · 2026Article
- Multifunctional copper-based metal-organic frameworks/chitosan derivative/exosome composite sponge for skin healing.iScience · 2025Article
- Synergistic Reduction of Breast Cancer Cell Viability and Aggressiveness Through Dual Inhibition of APE1 Redox Function and STAT3 Signaling.Cell biology international · 2025Article
- Article
- Preparation of liposomal nanocarriers containing all-trans retinoic acid and docetaxel and their evaluation in a lung cancer cell line.Scientific reports · 2025Article
- Inference and prediction for stochastic models of biological populations undergoing migration and proliferation.Journal of the Royal Society, Interface · 2025Review
- Multi-Omics and Molecular Docking Studies on Caffeine for Its Skin Rejuvenating Potentials.Pharmaceuticals (Basel, Switzerland) · 2025Article
- Surface-tailored graphene nanosheets targeting PI3K/Akt signaling of breast cancer cells.Scientific reports · 2025Article
19 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The scratch assay is an in vitro technique used to assess the contribution of molecular and cellular mechanisms to cell migration. The assay can also be used to evaluate therapeutic compounds before clinical use. Current quantification methods of scratch assays deal poorly with irregular cell-free areas and crooked leading edges which are features typically present in the experimental data. We introduce a new migration quantification method, called 'monolayer edge velocimetry', that permits analysis of low-quality experimental data and better statistical classification of migration rates than standard quantification methods. The new method relies on quantifying the horizontal component of the cell monolayer velocity across the leading edge. By performing a classification test on in silico data, we show that the method exhibits significantly lower statistical errors than standard methods. When applied to in vitro data, our method outperforms standard methods by detecting differences in the migration rates between different cell groups that the other methods could not detect. Application of this new method will enable quantification of migration rates from in vitro scratch assay data that cannot be analysed using existing methods.
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