ArticleJournal of the Royal Society, Interface2019
Collagen reorganization in cartilage under strain probed by polarization sensitive second harmonic generation microscopy.
Article in Journal of the Royal Society, Interface, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
49 citing papers in PubMed.
- Restoring pre-osteoarthritis cartilage matrix integrity via Col9 mRNA halts osteoarthritis progression.Science advances · 2026Article
- Second Harmonic Generation Imaging Links Collagen Structure and Biochemical Composition in Human Osteoarthritic Cartilage.Annals of biomedical engineering · 2026Article
- Collagen-Related Viscoelasticity as a Metric for Fracture Assessment in Cortical Bone.Annals of biomedical engineering · 2026Article
- Bioprinting of Microtissues Within Mechanically Tunable Support Baths to Engineer Anisotropic Musculoskeletal Tissues.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Collagen Mechanics.Sub-cellular biochemistry · 2026Review
- A Comprehensive Review of Auto- and Allogeneic Chondrocyte Transplantation in Animals and Humans From 1965 to 2025.Cartilage · 2025Article
- Characteristics of Lower Limb Dominant and Nondominant Joint Load Changes After Long-Distance Running in Young Male Runners Under OpenSim Environment.Sensors (Basel, Switzerland) · 2025Article
- Spatial Distribution of Extracellular Vesicles, Autofluorescence and CD9 Positivity Around Chondrocytes in the Superficial Layer of Articular Cartilage.Journal of extracellular vesicles · 2025Article
- Nanofibrous scaffolds for bone and cartilage regeneration.Applied physics reviews · 2025Article
- High-Speed Full-Color Polarized Light Imaging of Collagen Using a Polarization Camera.Bioengineering (Basel, Switzerland) · 2025Article
- Multiscale investigation of collagen structure in human skin and gel matrices using polarization resolved second harmonic generation microscopy.Scientific reports · 2025Article
- Restoring articular cartilage: insights from structure, composition and development.Nature reviews. Rheumatology · 2025Review
- Nonlinear Stress-Induced Transformations in Collagen Fibrillar Organization, Disorder and Strain Mechanisms in the Bone-Cartilage Unit.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Functional Scaffolds for Bone Tissue Regeneration: A Comprehensive Review of Materials, Methods, and Future Directions.Journal of functional biomaterials · 2024Review
- Cartilage Integrity: A Review of Mechanical and Frictional Properties and Repair Approaches in Osteoarthritis.Healthcare (Basel, Switzerland) · 2024Review
- Effects of mineralization on the hierarchical organization of collagen-a synchrotron X-ray scattering and polarized second harmonic generation study.Interface focus · 2024Article
- Investigating cartilage-related diseases by polarization-resolved second harmonic generation (P-SHG) imaging.APL bioengineering · 2024Article
- Functional Assessment of Human Articular Cartilage Using Second Harmonic Generation (SHG) Imaging: A Feasibility Study.Annals of biomedical engineering · 2024Article
- Article
- Categorization of collagen type I and II blend hydrogel using multipolarization SHG imaging with ResNet regression.Scientific reports · 2023Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Type II collagen fibril diameters in cartilage are beneath the diffraction limit of optical microscopy, which makes the assessment of collagen organization very challenging. In this work we use polarization sensitive second harmonic generation (P-SHG) imaging to map collagen organization in articular cartilage, addressing in particular its behaviour under strain and changes which occur in osteoarthritis. P-SHG yields two parameters, molecular order and orientation, which provide measures of the degree of organization both at the molecular scale (below the diffraction limit) and above a few hundred nanometres (at the image pixel size). P-SHG clearly demonstrates the zonal collagen architecture and reveals differences in the structure of the fibrils around chondrocytes. P-SHG also reveals sub-micron scale fibril re-organization in cartilage strips exposed to tensile loading, with an increase in local organization in the superficial zone which weakly correlates with tensile modulus. Finally, P-SHG is used to investigate osteoarthritic cartilage from total knee replacement surgery, and reveals widespread heterogeneity across samples both microscale fibril orientations and their sub-micron organization. By addressing collagen fibril structure on scales intermediate between conventional light and electron microscopy, this study provides new insights into collagen micromechanics and mechanisms of degradation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.