Evidence map›Paper›PMID 30958084›Full record

ArticleThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Society2019

Magnetically Promoted Rapid Immunofluorescence Staining for Frozen Tissue Sections.

Tatsuya Onishi, Sachiko Matsuda, Yuki Nakamura, Junko Kuramoto, Akinori Tsuruma, Satoshi Sakamoto, Shunichi Suzuki, Daiichiro Fuchimoto, Akira Onishi, Shinichi Chikaki and 16 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.9field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 12 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

26 authors at 13 institutions in 1 country.

Tatsuya OnishiDepartment of Surgery, Keio University School of Medicine, Tokyo, Japan.
Sachiko MatsudaDepartment of Surgery, Keio University School of Medicine, Tokyo, Japan.
Yuki NakamuraDepartment of Surgery, Keio University School of Medicine, Tokyo, Japan.
Junko KuramotoDepartment of Pathology, Keio University School of Medicine, Tokyo, Japan.
Akinori TsurumaSchool of Life Science and Technology, Tokyo Institute of Technology, Yokohama, Japan.
Satoshi SakamotoSchool of Life Science and Technology, Tokyo Institute of Technology, Yokohama, Japan.
Shunichi SuzukiDivision of Animal Sciences, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization, Tsukuba, Japan.
Daiichiro FuchimotoDivision of Animal Sciences, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization, Tsukuba, Japan.
Akira OnishiLaboratory of Animal Reproduction, Department of Animal Science and Resources, College of Bioresource Sciences, Nihon University, Fujisawa, Japan.
Shinichi ChikakiGraduate School of Engineering, The University of Tokyo, Tokyo, Japan.
Miki KanekoGraduate School of Engineering, The University of Tokyo, Tokyo, Japan.
Akihiro KuwahataGraduate School of Engineering, The University of Tokyo, Tokyo, Japan.
Masaki SekinoGraduate School of Engineering, The University of Tokyo, Tokyo, Japan.
Hiroshi YasunoTamagawa Seiki Co. Ltd., Nagano, Japan.
Naohiro HanyuTamagawa Seiki Co. Ltd., Nagano, Japan.
Tomoko KuritaDepartment of Breast Surgery, Nippon Medical School, Tokyo, Japan.
Hiroyuki TakeiDepartment of Breast Surgery, Nippon Medical School, Tokyo, Japan.
Takashi SakataniDepartment of Diagnostic Pathology, Nippon Medical School Hospital, Tokyo, Japan.
Kanae TarunoDivision of Breast Surgical Oncology, Showa University, Tokyo, Japan.
Seigo NakamuraDivision of Breast Surgical Oncology, Showa University, Tokyo, Japan.
Tetsu HayashidaDepartment of Surgery, Keio University School of Medicine, Tokyo, Japan.
Hiromitsu JinnoDepartment of Surgery, Teikyo University School of Medicine, Tokyo, Japan.
Moriaki KusakabeGraduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
Hiroshi HandaDepartment of Nanoparticle Translational Research, Tokyo Medical University, Tokyo, Japan.
Kaori KameyamaDepartment of Diagnostic Pathology, Keio University Hospital, Tokyo, Japan.
Yuko KitagawaDepartment of Surgery, Keio University School of Medicine, Tokyo, Japan.
Keio University · JPThe University of Tokyo · JPNippon Medical School · JPNippon Seiki (Japan) · JPShowa University · JPTokyo Institute of Technology · JPInstitute of Agrobiological Sciences · JPKeio University Hospital · JPNational Agriculture and Food Research Organization · JPNihon University · JPNippon Medical School Hospital · JPTeikyo University · JPTokyo Medical University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Current immunohistochemistry methods for diagnosing abnormal cells, such as cancer cells, require multiple steps and can be relatively slow compared with intraoperative frozen hematoxylin and eosin staining, and are therefore rarely used for intraoperative examination. Thus, there is a need for novel rapid detection methods. We previously demonstrated that functionalized fluorescent ferrite beads (FF beads) magnetically promoted rapid immunoreactions. The aim of this study was to improve the magnetically promoted rapid immunoreaction method using antibody-coated FF beads and a magnet subjected to a magnetic field. Using frozen sections of xenograft samples of A431 human epidermoid cancer cells that express high levels of epidermal growth factor receptor (EGFR) and anti-EGFR antibody-coated FF beads, we reduced the magnetically promoted immunohistochemistry procedure to a 1-min reaction and 1-min wash. We also determined the optimum magnetic force for the antibody reaction (from 7.79 × 10

Indexed as

Frozen SectionsMagnetic PhenomenaAnimalsAntibodies, NeutralizingCell Line, TumorFluorescent Antibody TechniqueHumansLymphatic MetastasisStaining and LabelingSwineTime FactorsAntibodies, Neutralizingcancerfluorescent ferrite beadsimmunostainingintraoperative diagnosismagnetic field

Identifiers

PMID30958084
PMCPMC6669860
OpenAlexW2935974918

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.