ArticleJournal of neuroscience research2020
Step-function luminopsins for bimodal prolonged neuromodulation.
Article in Journal of neuroscience research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 35 citations in OpenAlex.
- A bioluminescent activity dependent platform, BLADe, for converting intracellular activity to photoreceptor activation.Scientific reports · 2026Article
- A Bioluminescent Activity Dependent (BLADe) Platform for Converting Intracellular Activity to Photoreceptor Activation.bioRxiv : the preprint server for biology · 2025Article
- Strength of activation and temporal dynamics of bioluminescent-optogenetics in response to systemic injections of the luciferin.NeuroImage · 2024Article
- Recent advances in bioluminescent probes for neurobiology.Neurophotonics · 2024Article
- Bioluminescence as a functional tool for visualizing and controlling neuronal activityNeurophotonics · 2024Article
- Efficient opto- and chemogenetic control in a single molecule driven by FRET-modified bioluminescence.Neurophotonics · 2024Article
- Engineering luminopsins with improved coupling efficiencies.Neurophotonics · 2024Article
- Understanding neural circuit function through synaptic engineering.Nature reviews. Neuroscience · 2024Review
- Engineering luminopsins with improved coupling efficiencies.bioRxiv : the preprint server for biology · 2023Article
- Coelenterazine-Type Bioluminescence-Induced Optical Probes for Sensing and Controlling Biological Processes.International journal of molecular sciences · 2023Review
- Hybrid fiber optic-fMRI for multimodal cell-specific recording and manipulation of neural activity in rodents.Neurophotonics · 2022Review
- Review
- Selective control of synaptically-connected circuit elements by all-optical synapses.Communications biology · 2022Article
- Rhodopsins: An Excitingly Versatile Protein Species for Research, Development and Creative Engineering.Frontiers in chemistry · 2022Review
- Bioluminescence-Optogenetics: A Practical Guide.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Applications of Bioluminescence-Optogenetics in Rodent Models.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Restoring Function After Severe Spinal Cord Injury Through BioLuminescent-OptoGenetics.Frontiers in neurology · 2021Article
- Bioluminescence-Driven Optogenetics.Life (Basel, Switzerland) · 2020Review
- Minimally invasive neural stimulation with a novel ultra-sensitive step function opsin: implications and future directions.Journal of neurophysiology · 2020Article
- Seeing (and Using) the Light: Recent Developments in Bioluminescence Technology.Cell chemical biology · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Although molecular tools for controlling neuronal activity by light have vastly expanded, there are still unmet needs which require development and refinement. For example, light delivery into the brain is still a major practical challenge that hinders potential translation of optogenetics in human patients. In addition, it would be advantageous to manipulate neuronal activity acutely and precisely as well as chronically and non-invasively, using the same genetic construct in animal models. We have previously addressed these challenges by employing bioluminescence and have created a new line of opto-chemogenetic probes termed luminopsins by fusing light-sensing opsins with light-emitting luciferases. In this report, we incorporated Chlamydomonas channelrhodopsin 2 with step-function mutations as the opsin moiety in the new luminopsin fusion protein termed step-function luminopsin (SFLMO). Bioluminescence-induced photocurrent lasted longer than the bioluminescence signal due to very slow deactivation of the mutated channel. In addition, bioluminescence was able to activate most of the channels on the cell surface due to the extremely high light sensitivity of the channel. This efficient channel activation was partly mediated by radiationless bioluminescence resonance energy transfer due to the proximity of luciferase and opsin. To test the utility of SFLMOs in vivo, we transduced the substantia nigra unilaterally via a viral vector in male rats. Injection of the luciferase substrate as well as conventional photostimulation via fiber optics elicited circling behaviors. Thus, SFLMOs expand the current approaches for manipulation of neuronal activity in the brain and add more versatility and practicality to optogenetics in freely behaving animals.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.