ArticleTranslational medicine communications2018
The comparative effects of high fat diet or disturbed blood flow on glycocalyx integrity and vascular inflammation.
Article in Translational medicine communications, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 31 citations in OpenAlex.
- Western diet-induced endothelial glycocalyx remodeling is partially reversed by fasting.American journal of physiology. Heart and circulatory physiology · 2025Article
- Time course and contributors to greater glycocalyx thickness and integrity following Western diet consumption.American journal of physiology. Heart and circulatory physiology · 2025Article
- Engineering Mechanical Microenvironments: Integration of Substrate and Flow Mechanics Reveals the Impact on the Endothelial Glycocalyx.ACS biomaterials science & engineering · 2025Article
- Mechanisms of triple-negative breast cancer extravasation: Impact of the physical environment and endothelial glycocalyx.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2024Review
- Lack of Laminar Shear Stress Facilitates the Endothelial Uptake of Very Small Superparamagnetic Iron Oxide Nanoparticles by Modulating the Endothelial Surface Layer.International journal of nanomedicine · 2024Article
- Endothelial mechanobiology in atherosclerosis.Cardiovascular research · 2023Review
- The role of hyaluronan in endothelial glycocalyx and potential preventative lifestyle strategy with advancing age.Current topics in membranes · 2023Article
- New developments in translational microcirculatory research.American journal of physiology. Heart and circulatory physiology · 2022Review
- Empagliflozin mitigates endothelial inflammation and attenuates endoplasmic reticulum stress signaling caused by sustained glycocalyx disruption.Scientific reports · 2022Article
- Microcirculatory and glycocalyx properties are lowered by high-salt diet but augmented by Western diet in genetically heterogeneous mice.American journal of physiology. Heart and circulatory physiology · 2022Article
- Disturbed flow's impact on cellular changes indicative of vascular aneurysm initiation, expansion, and rupture: A pathological and methodological review.Journal of cellular physiology · 2022Review
- Paracrine Shear-Stress-Dependent Signaling from Endothelial Cells Affects Downstream Endothelial Function and Inflammation.International journal of molecular sciences · 2021Article
- Inflammation and enhanced atherogenesis in the carotid artery with altered blood flow in an atherosclerosis-resistant mouse strain.Physiological reports · 2021Article
- Endothelial Glycocalyx-Mediated Intercellular Interactions: Mechanisms and Implications for Atherosclerosis and Cancer Metastasis.Cardiovascular engineering and technology · 2021Review
- High Fat Diet Dysbiotic Mechanism of Decreased Gingival Blood Flow.Frontiers in physiology · 2021Article
- Targeted Intravenous Nanoparticle Delivery: Role of Flow and Endothelial Glycocalyx Integrity.Annals of biomedical engineering · 2020Article
- Label-free photoacoustic and ultrasound imaging for murine atherosclerosis characterization.APL bioengineering · 2020Article
- Flow-regulated endothelial glycocalyx determines metastatic cancer cell activity.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2020Article
- Endothelial responses to shear stress in atherosclerosis: a novel role for developmental genes.Nature reviews. Cardiology · 2020Review
- Blood-Brain Barrier Damage in Ischemic Stroke and Its Regulation by Endothelial Mechanotransduction.Frontiers in physiology · 2020Review
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 1 country.
Funding
Abstract
BACKGROUND AND
aimsEndothelial surface glycocalyx shedding plays a role in endothelial dysfunction and increases vessel wall permeability, which can lead to inflammation and atherogenesis. We sought to elucidate whether a high fat diet (HFD) or disturbed blood flow conditions, both of which are atherogenic risk factors, would contribute more detrimentally to pre-atherosclerotic loss of endothelial glycocalyx integrity and vascular inflammation.
methodsSix to seven week-old C57BL/6-background apolipoprotein-E-knockout (ApoE-KO) male mice were either fed a chow diet, fed a modified Western HFD, and/or subjected to a partial left carotid artery (LCA) ligation procedure to induce disturbed blood flow patterns in the LCA. Mice were sacrificed after 1 week of experimental conditions. Both LCA and right carotid artery (RCA) vessels were dissected and preserved to compare glycocalyx coverage and thickness as well as macrophage accumulation in carotid arterial walls amongst and between cohorts.
resultsGlycocalyx coverage of the endothelium was significantly reduced in the LCAs of HFD fed mice when compared to the control. More significant reduction in glycocalyx coverage occurred in the LCAs of mice exposed to disturbed flow by partial LCA ligation when compared to the control. No differences were found in glycocalyx coverage of RCAs from all cohorts. Regarding inflammation, no difference in macrophage accumulation in carotid arterial walls was observed when comparing the LCAs and RCAs of control and HFD fed mice. However, macrophage infiltration in vessel walls showed a 20-fold increase in the LCAs exposed to disturbed flow following ligation, when compared to control LCAs, while no such statistical difference was observed between the RCAs of the group.
conclusionsIn our mouse model, endothelial glycocalyx integrity was compromised more by disturbed blood flow patterns than by exposure of the carotid vessel to HFD conditions. The pathophysiological implications include endothelial dysfunction, which correlates to macrophage infiltration in vessel walls and promotes atherogenesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.