ArticleFood science and biotechnology2019
Rapid on-site detection of shrimp allergen tropomyosin using a novel ultrafast PCR system.
Article in Food science and biotechnology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 26 citations in OpenAlex.
- Ultrafast PCR assay for identification of three king crabs-Food science and biotechnology · 2025Article
- Enzyme Engineering: Performance Optimization, Novel Sources, and Applications in the Food Industry.Foods (Basel, Switzerland) · 2024Review
- Ultrasensitive Quantification of Crustacean Tropomyosin by Immuno-PCR.International journal of molecular sciences · 2023Article
- Detection method for genetically modified potato using an ultra-fast PCR system.Food science and biotechnology · 2023Article
- Rapid On-Site Identification for Three Arcidae Species (Foods (Basel, Switzerland) · 2022Article
- Aptamer-Based Fluorescent Biosensor for the Rapid and Sensitive Detection of Allergens in Food Matrices.Foods (Basel, Switzerland) · 2021Review
- Multisyringe Flow Injection Analysis of Tropomyosin Allergens in Shellfish Samples.Molecules (Basel, Switzerland) · 2021Article
- Electrochemiluminescence immunosensor for tropomyosin using carbon nanohorns/Nafion/FeMikrochimica acta · 2020Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Shrimp is seafood that can commonly trigger allergic reactions. In this study, the ultrafast real-time PCR assay with portable device was developed to detect a shrimp-derived major allergen, tropomyosin, without complicated DNA extraction. For shrimp allergen detection, a specific primer pair was designed based on the shrimp tropomyosin gene and 18S ribosomal RNA gene as internal control. Primer specificity was assessed using 8 common seafood species. Serially diluted shrimp DNA was used to determine the limit of detection of the ultrafast PCR system, which was approximately 3.2 pg. Twenty-three food samples containing shrimp were evaluated to verify the applicability of a direct ultrafast PCR method for detecting shrimp allergens without DNA isolation. It took less than 30 min from sample preparation-to-result analysis to detect shrimp DNA in raw and processed samples. Therefore, this PCR system can be effectively and conveniently utilized in the field to detect shrimp in various food products.
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