ArticleJournal of parasitic diseases : official organ of the Indian Society for Parasitology2019
Development and comparison of real-time and conventional PCR tools targeting β-tubulin gene for detection of
Article in Journal of parasitic diseases : official organ of the Indian Society for Parasitology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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4 citing papers in PubMed, 14 citations in OpenAlex.
- Establishment and application of a loop-mediated isothermal amplification method based onFrontiers in veterinary science · 2025Article
- Recent advances in the biosensors application for reviving infectious disease management in silkworm model: a new way to combat microbial pathogens.Archives of microbiology · 2024Review
- Adaptation of the classical end-point ITS-PCR for the diagnosis of avian trichomonosis to a real-time PCR reveals Bonelli's eagle as a new host for Trichomonas gypaetinii.Parasitology research · 2022Article
- Investigation on Pathological Aspects, Mode of Transmission, and Tissue Tropism of Antheraea proylei Nucleopolyhedrovirus Infecting Oak Tasar Silkworm.Journal of insect science (Online) · 2022Article
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Authors and funding
5 authors at 1 institution in 1 country.
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Abstract
Microsporidiosis (Pebrine) caused by the microsporidian parasite is one of the important devastating disease which affect the silk production leading to an unprofitable harvest. Till date ribosomal RNA (rRNA) gene was used as a target for detection of microsporidian species. In this study, we describe conventional and SYBR green based real-time PCR techniques alternatively targeting β-tubulin gene for quantitative detection of microsporidia infecting both the mulberry and non-mulberry silkworms. The modified DNA extraction method followed in our study was found to be easy, economical and could be used for both conventional and real time PCR as template. The real time qPCR revealed the expression of β-tubulin gene in different infected tissues of the silkworm
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