ArticleJournal of food science and technology2019
A sensitive multiplex PCR protocol for simultaneous detection of chicken, duck, and pork in beef samples.
Article in Journal of food science and technology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Development of Rapid Alkaline Lysis-Polymerase Chain Reaction Technique for Authentication of Mithun (Molecules (Basel, Switzerland) · 2025Article
- Identification of meat adulteration in minced meat samples labeled as beef and mutton in Tehran stores using duplex PCR.Food science & nutrition · 2024Article
- Application of machine learning approach on halal meat authentication principle, challenges, and prospects: A review.Heliyon · 2024Review
- Article
- Lateral flow immunoassay-based absolute point-of-care technique for authentication of meat and commercial meat products.Journal of food science and technology · 2023Article
- Heat-Treated Meat Origin Tracing and Authenticity through a Practical Multiplex Polymerase Chain Reaction Approach.Nutrients · 2022Article
- Visual Detection of Chicken Adulteration Based on a Lateral Flow Strip-PCR Strategy.Foods (Basel, Switzerland) · 2022Article
- Development of loop-mediated isothermal method and comparison with conventional PCR assay for rapid on spot identification of tissue of cattle origin.Journal of food science and technology · 2021Article
- A quantitative method for detecting meat contamination based on specific polypeptides.Animal bioscience · 2021Article
- A Practical Approach to Identifying Processed White Meat of Guinea Fowl, Rabbit, and Selected Fish Species Using End-Point PCR.International journal of food science · 2021Article
- Recombinase Polymerase Amplification Based Multiplex Lateral Flow Dipstick for Fast Identification of Duck Ingredient in Adulterated Beef.Animals : an open access journal from MDPI · 2020Article
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A rapid and sensitive multiplex PCR assay was developed for simultaneous identification of the adulteration ingredients of chicken, duck and pork in beef. Specific primers for the mitochondrial genes of Cyt b, CO III, ATPase subunit 8/6 and Cyt b of chicken, duck, pork, and beef, respectively, were adopted in the assay. DNA exaction from meat samples was carried out by using magnetic nanoparticles as rapid separation substrates. The multiplex PCR assay showed that the limit of detection was 0.05% for each species. Moreover, the multiplex PCR specifically identified five beef samples adulterated with pork and one beef samples adulterated with chicken among the 35 commercial samples examined, indicating the practicability of this multiplex PCR method for identifying adulterated ingredients of chicken, duck, and pork in commercial beef products.
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