ArticlePlanta2019
Overexpression of a basic helix-loop-helix transcription factor gene, SlbHLH22, promotes early flowering and accelerates fruit ripening in tomato (Solanum lycopersicum L.).
Article in Planta, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 51 citations in OpenAlex.
- The carotenoids metabolism during tomato fruit ripening: insights into multi-level regulatory network.BMC plant biology · 2026Review
- Genome- and Transcriptome-Wide Characterization and Expression Analyses of bHLH Transcription Factor Family Reveal Their Relevance to Salt Stress Response in Tomato.Plants (Basel, Switzerland) · 2025Article
- Transcriptome analyses reveal the flowering regulatory networks in the desert ephemeral plantFrontiers in plant science · 2025Article
- Tomato activates ethylene signaling to maintainFrontiers in plant science · 2025Article
- SlUPA-like, a bHLH Transcription Factor in Tomato (International journal of molecular sciences · 2024Article
- The transcription factor PbbHLH164 is destabilized by PbRAD23C/D.1 and mediates ethylene biosynthesis during pear fruit ripening.Journal of advanced research · 2024Article
- Comparative transcriptomic analysis of the nodulation-competent zone and inference of transcription regulatory network in silicon applied Glycine max [L.]-Merr. Roots.Plant cell reports · 2024Article
- A tomato NAC transcription factor, SlNAP1, directly regulates gibberellin-dependent fruit ripening.Cellular & molecular biology letters · 2024Article
- Transcriptional regulation of tomato fruit ripening.Physiology and molecular biology of plants : an international journal of functional plant biology · 2024Review
- Abscission zone metabolism impacts pre- and post-harvest fruit quality: a very attaching story.Frontiers in plant science · 2024Review
- Article
- Understanding Transcription Factors and How They Affect Processes in Cucumber Sex Determination.Metabolites · 2023Article
- Genome-wide identification, expression analysis, and potential roles under low-temperature stress of bHLH gene family inFrontiers in plant science · 2023Article
- A comparative transcriptomics and eQTL approach identifies SlWD40 as a tomato fruit ripening regulator.Plant physiology · 2022Article
- Integrative Analyses of Transcriptomes and Metabolomes Reveal Associated Genes and Metabolites with Flowering Regulation in Common Vetch (International journal of molecular sciences · 2022Article
- CabHLH79 Acts Upstream ofInternational journal of molecular sciences · 2022Article
- Atlas of phenotypic, genotypic and geographical diversity present in the European traditional tomato.Horticulture research · 2022Article
- Article
- Genome-Wide Identification and Characterization of MelonPlants (Basel, Switzerland) · 2021Article
- The DUF221 domain-containing (DDP) genes identification and expression analysis in tomato under abiotic and phytohormone stress.GM crops & food · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MAIN
conclusionThe overexpression of SlbHLH22 functioned in controlling flowering time, accelerated fruit ripening, and produced more ethylene-producing phenotypes in tomato. Flowering and fruit ripening are two complex transition processes regulated by various internal and external factors that ultimately lead to fruit maturation and final seed dispersal. The basic helix-loop-helix (bHLH) transcription factor is the largest TF gene family in plants that controls various biological and developmental aspects, but the actual roles of these genes have not been fully studied. Here, we performed a functional characterization of the bHLH gene SlbHLH22 in tomato. SlbHLH22 was fully expressed in tomato flowers, while a moderate expression level was also observed in fruits at different developmental stages. Overexpression of the SlbHLH22 gene revealed that it is highly involved in controlling flowering time, through the activation of the SlSFT or SlLFY genes, and promoting fruit ripening and improved carotenoid accumulation. The expression patterns of carotenoid-related genes (SlPYS1) were also upregulated in transgenic tomato fruits. In transgenic tomato fruit, we observed clear changes in colour from green to orange with enhanced expression of the SlbHLH22 gene. SlbHLH22 was upregulated under exogenous ACC, IAA, ABA, and ethephon. Overexpression of SlbHLH22 also promotes ethylene production. Moreover, ethylene biosynthesis and perception genes (SlACO3, SlACS1, SlACS2, SlACS4, SlACS1a, SlEIN1, SlEIN2, SlEIN3, SlEIN4, SlETR2, SlETR3, SlSAM3, and SlSAMS) were upregulated. Ripening-related genes (SlAP2a, SlCNR, SlNOR, SlMYB, and SlTAG) were consistent in their expression pattern in transgenic plants. Finally, our study provides evidence that tomato bHLH genes play an important role in flowering, fruit ripening, and development.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.