ArticleWater research2019
Evaluation of multiple laboratory performance and variability in analysis of recreational freshwaters by a rapid Escherichia coli qPCR method (Draft Method C).
Article in Water research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed.
- Public health use and lessons learned from a statewide SARS-CoV-2 wastewater monitoring program (MiNET).Heliyon · 2024Article
- Fecal Impairment Framework, A New Conceptual Framework for Assessing Fecal Contamination in Recreational Waters.Environmental management · 2024Article
- Interlaboratory performance and quantitative PCR data acceptance metrics for NIST SRM® 2917.Water research · 2022Article
- Bacterial and viral fecal indicator predictive modeling at three Great Lakes recreational beach sites.Water research · 2022Article
- Performance of NIST SRM® 2917 with 13 recreational water quality monitoring qPCR assays.Water research · 2022Article
- Large-scale comparison of E. coli levels determined by culture and a qPCR method (EPA Draft Method C) in Michigan towards the implementation of rapid, multi-site beach testing.Journal of microbiological methods · 2021Article
- A short history of methods used to measure bathing beach water quality.Journal of microbiological methods · 2021Review
- Absolute quantification of E. coli virulence and housekeeping genes to determine pathogen loads in enumerated environmental samples.PloS one · 2021Article
- Microbial Indicators of Fecal Pollution: Recent Progress and Challenges in Assessing Water Quality.Current environmental health reports · 2020Review
- Article
- Standardized data quality acceptance criteria for a rapid Escherichia coli qPCR method (Draft Method C) for water quality monitoring at recreational beaches.Water research · 2019Article
Corrections and comments
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Authors and funding
20 authors.
Funding
Abstract
There is interest in the application of rapid quantitative polymerase chain reaction (qPCR) methods for recreational freshwater quality monitoring of the fecal indicator bacteria Escherichia coli (E. coli). In this study we determined the performance of 21 laboratories in meeting proposed, standardized data quality acceptance (QA) criteria and the variability of target gene copy estimates from these laboratories in analyses of 18 shared surface water samples by a draft qPCR method developed by the U.S. Environmental Protection Agency (EPA) for E. coli. The participating laboratories ranged from academic and government laboratories with more extensive qPCR experience to "new" water quality and public health laboratories with relatively little previous experience in most cases. Failures to meet QA criteria for the method were observed in 24% of the total 376 test sample analyses. Of these failures, 39% came from two of the "new" laboratories. Likely factors contributing to QA failures included deviations in recommended procedures for the storage and preparation of reference and control materials. A master standard curve calibration model was also found to give lower overall variability in log
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