Evidence map›Paper›PMID 30953394›Full record

ArticleCell proliferation2019

Human dental pulp stem cells regulate allogeneic NK cells' function via induction of anti-inflammatory purinergic signalling in activated NK cells.

Fei Yan, Ousheng Liu, Haixia Zhang, Yueying Zhou, Dian Zhou, Zekun Zhou, Yuhong He, Zhangui Tang, Songlin Wang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell proliferation, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
1.0field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 24 citations in OpenAlex.

  1. Trial
  2. Review
  3. Review
  4. Review
  5. Therapeutic potential of CD73Histology and histopathology · 2025
    Review
  6. Review
  7. Review
  8. Article
  9. Review
  10. Review
  11. Article
  12. Review
  13. Review
  14. Article
  15. Therapeutic Functions of Stem Cells from Oral Cavity: An Update.International journal of molecular sciences · 2020
    Review
  16. Article
  17. Review
  18. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Fei YanXiangya Stomatological Hospital and School of Stomatology, Central South University, Changsha, Hunan, China.ORCID https://orcid.org/0000-0002-3304-4718
Ousheng LiuXiangya Stomatological Hospital and School of Stomatology, Central South University, Changsha, Hunan, China.
Haixia ZhangDepartment of Oncology, The Second Xiangya Hospital, Central South University, Changsha, Hunan, China.
Yueying ZhouXiangya Stomatological Hospital and School of Stomatology, Central South University, Changsha, Hunan, China.
Dian ZhouXiangya Stomatological Hospital and School of Stomatology, Central South University, Changsha, Hunan, China.
Zekun ZhouXiangya Stomatological Hospital and School of Stomatology, Central South University, Changsha, Hunan, China.
Yuhong HeXiangya Stomatological Hospital and School of Stomatology, Central South University, Changsha, Hunan, China.
Zhangui TangXiangya Stomatological Hospital and School of Stomatology, Central South University, Changsha, Hunan, China.
Songlin WangBeijing Key Laboratory of Tooth Regeneration and Function Reconstruction, Capital Medical University School of Stomatology, Beijing, China.
Central South University · CNCapital Medical University · CN

Funding

Health and Family Planning Commission of Hunan Province 20180264National Natural Science Foundation of China 81300841National Natural Science Foundation of Hunan Province 2017JJ2341National Natural Science Foundation of Hunan Province 2017JJ3461
6 · The paper itself

Abstract

objectivesMesenchymal stem cells (MSCs) could regulate the function of various immune cells. It remains unclear whether MSCs additionally possess immunostimulatory properties. We investigated the impact of human MSCs on the responsiveness of primary natural killer (NK) cells in terms of induction of anti-inflammatory purinergic signalling. MATERIAL AND

methodsWe obtained human bone marrow mesenchymal stem cells (BMMSCs) and dental pulp stem cells (DPSCs). NK cells were isolated from peripheral blood of healthy volunteers. Activated NK cells were cultured with MSCs. Proliferation assay, apoptosis analysis, activating or inhibitory receptor expression and degranulation assay were used to explore NK cells' function. High-performance liquid chromatography was used to investigate the purinergic signalling in activated NK cells.

resultsBoth DPSCs and BMMSCs could impair proliferation and promote apoptosis of activated NK cells. Also, activated NK cells could cause DPSCs to lyse. Furthermore, the expression of activating NK cells' receptors was decreased, but inhibitory receptors of NK cells were elevated following co-cultivation. NK cells acquired CD73 expression, while MSCs could release ATP into the extracellular space where nucleotides were converted into adenosine (ADO) following co-culture system. Under the existence of exogenous 2-chloroadenosine (CADO), the cytotoxic capacity of NK cells was remarkably depressed in a concentration-dependent manner.

conclusionsDPSCs and BMMSCs could depress NK cells' function by hydrolysing ATP to ADO using CD39 and CD73 enzymatic activity. Our data suggested that DPSCs might represent a new strategy for treating immune-related diseases by regulating previously unrecognized functions in innate immune responses.

Indexed as

2-Chloroadenosine5'-NucleotidaseApoptosisCell ProliferationCoculture TechniquesCytotoxicity, ImmunologicDental PulpGPI-Linked ProteinsHumansInflammation MediatorsK562 CellsKiller Cells, NaturalLymphocyte ActivationMesenchymal Stem CellsPurinesReceptors, Natural Killer Cell2-Chloroadenosine5'-NucleotidaseGPI-Linked ProteinsInflammation MediatorsNT5E protein, humanPurinesReceptors, Natural Killer CelladenosineCD73dental pulp stem cellsimmunoregulatorynatural killer cellspurinergic signalling

Identifiers

PMID30953394
PMCPMC6536423
OpenAlexW2935486854

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.