ArticleScientific reports2019
Tolerance of the freeze-dried mouse sperm nucleus to temperatures ranging from -196 °C to 150 °C.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed.
- Stallion Sperm Lyophilisation: Effect of Source of Sperm and Reproductive Season.Veterinary sciences · 2026Article
- Monosodium glutamate protects DNA of freeze-dried somatic cells and improves development of somatic cell nuclear transfer embryos.The Journal of reproduction and development · 2026Article
- The challenges and prospects of mammalian reproduction in extraterrestrial environments.The Journal of reproduction and development · 2026Review
- EV-Mediated Oncogenic Regulation in Lung Cancer and Clinical Translation: From Liquid Biopsy to Targeted Delivery Systems.International journal of nanomedicine · 2026Review
- Freeze-Drying and Room Temperature Preservation of Mammalian Sperm.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Improved birth rates via rehydration of mouse freeze-dried spermatozoa using high-temperature ultrapure water.PloS one · 2026Article
- Birth of offspring derived from cryopreserved rat sperm after shipment in a Styrofoam box at -80°C.Experimental animals · 2025Article
- Monosodium glutamate enhances freeze-dried mouse sperm quality during room-temperature storage.Scientific reports · 2025Article
- Can Humanity Thrive Beyond the Galaxy?The Journal of reproduction and development · 2025Review
- Extracting and analyzing micronuclei from mouse two-cell embryos fertilized with freeze-dried spermatozoa.Communications biology · 2025Article
- Method for long-term room temperature storage of mouse freeze-dried sperm.Scientific reports · 2025Article
- Effects of trehalose supplementation on Pesisir bull sperm quality during storage.Open veterinary journal · 2025Article
- Production of Healthy Mice from Freeze-Dried Spermatozoa Preserved at Room Temperature.Methods in molecular biology (Clifton, N.J.) · 2025Article
- High-concentration bovine serum albumin enhances fertilization ability of cold-stored rat sperm.The Journal of reproduction and development · 2024Article
- A novel, simplified method to prepare and preserve freeze-dried mouse sperm in plastic microtubes.The Journal of reproduction and development · 2023Article
- Production of mouse offspring from zygotes fertilized with freeze-dried spermatids.Scientific reports · 2022Article
- Production of offspring from vacuum-dried mouse spermatozoa and assessing the effect of drying conditions on sperm DNA and embryo development.The Journal of reproduction and development · 2022Article
- Healthy cloned offspring derived from freeze-dried somatic cells.Nature communications · 2022Article
- Small extracellular vesicles isolation and separation: Current techniques, pending questions and clinical applications.Theranostics · 2022Review
- Mailing viable mouse freeze-dried spermatozoa on postcards.iScience · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It has long been believed that tolerance against extreme environments is possible only for 'lower' groups, such as archaea, bacteria or tardigrades, and not for more 'advanced' species. Here, we demonstrated that the mammalian sperm nucleus also exhibited strong tolerance to cold and hot temperatures. When mouse spermatozoa were freeze-dried (FD), similar to the anhydrobiosis of Tardigrades, all spermatozoa were ostensibly dead after rehydration. However, offspring were obtained from recovered FD sperm nuclei, even after repeated treatment with conditions from liquid nitrogen to room temperature. Conversely, when FD spermatozoa were heated at 95 °C, although the birth rate was decreased with increasing duration of the treatment, offspring were obtained even for FD spermatozoa that had been heat-treated for 2 h. This period was improved up to 6 h when glucose was replaced with trehalose in the freeze-drying medium, and the resistance temperature was extended up to 150 °C for short periods of treatment. Randomly selected offspring grew into healthy adults. Our results suggest that, when considering the sperm nucleus/DNA as the material that is used as a blueprint of life, rather than cell viability, a significant tolerance to extreme temperatures is present even in 'higher' species, such as mammals.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.