ArticleNature communications2019
A fungal substrate mimicking molecule suppresses plant immunity via an inter-kingdom conserved motif.
Article in Nature communications, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers, 1 of them a synthesis that pooled it.
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Who cites it
38 citing papers in PubMed, 1 synthesis or guideline pooled it, 80 citations in OpenAlex.
- The front line of defence: a meta-analysis of apoplastic proteases in plant immunity.Journal of experimental botany · 2021Pooled it
- A co-evolved peptide-GPCR system senses host entry to drive fungal infection.Nature communications · 2026Article
- NbRD21 protease controls receptor kinase homeostasis in Nicotiana benthamiana.The New phytologist · 2026Article
- Processing and release of the maize phytocytokine Zip1.Plant physiology · 2026Article
- SsPit2A/B Effectors fromPlants (Basel, Switzerland) · 2026Article
- TurboID-Based Proximity Labeling for Exploiting Effector Interacting Proteins in the Fungal Maize Pathogen Ustilago maydis.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Apoplastic Fluid Collection/Analysis of Ustilago maydis-Infected Maize Leaves.Methods in molecular biology (Clifton, N.J.) · 2026Article
- RD21-like proteases: key effector hubs in plant-pathogen interactions.Journal of experimental botany · 2025Review
- Silenced cutters: mechanisms and effects of protease inhibition in plant-pathogen interactions.Journal of experimental botany · 2025Review
- NbPIRIN promotes the protease activity of papain-like cysteine protease NbRD21 to inhibit Chinese wheat mosaic virus infection.PLoS pathogens · 2025Article
- A catalogue of virulence strategies mediated by phytopathogenic effectors.Fundamental research · 2025Review
- APlants (Basel, Switzerland) · 2025Article
- Fungal evasion ofProceedings of the National Academy of Sciences of the United States of America · 2025Article
- The E3 ligase OsPUB33 controls rice grain size and weight by regulating the OsNAC120-BG1 module.The Plant cell · 2024Article
- A perspective on varied fungal virulence factors causing infection in host plants.Molecular biology reports · 2024Review
- NIa-Pro of sugarcane mosaic virus targets Corn Cysteine Protease 1 (CCP1) to undermine salicylic acid-mediated defense in maize.PLoS pathogens · 2024Article
- XYLEM CYSTEINE PEPTIDASE 1 and its inhibitor CYSTATIN 6 regulate pattern-triggered immunity by modulating the stability of the NADPH oxidase RESPIRATORY BURST OXIDASE HOMOLOG D.The Plant cell · 2024Article
- A transcriptional activator effector of Ustilago maydis regulates hyperplasia in maize during pathogen-induced tumor formation.Nature communications · 2023Article
- Ustilago maydis PR-1-like protein has evolved two distinct domains for dual virulence activities.Nature communications · 2023Article
- Combination of in vivo proximity labeling and co-immunoprecipitation identifies the host target network of a tumor-inducing effector in the fungal maize pathogen Ustilago maydis.Journal of experimental botany · 2023Article
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11 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ustilago maydis is a biotrophic fungus causing corn smut disease in maize. The secreted effector protein Pit2 is an inhibitor of papain-like cysteine proteases (PLCPs) essential for virulence. Pit2 inhibitory function relies on a conserved 14 amino acids motif (PID14). Here we show that synthetic PID14 peptides act more efficiently as PLCP inhibitors than the full-length Pit2 effector. Mass spectrometry shows processing of Pit2 by maize PLCPs, which releases an inhibitory core motif from the PID14 sequence. Mutational analysis demonstrates that two conserved residues are essential for Pit2 function. We propose that the Pit2 effector functions as a substrate mimicking molecule: Pit2 is a suitable substrate for apoplastic PLCPs and its processing releases the embedded inhibitor peptide, which in turn blocks PLCPs to modulate host immunity. Remarkably, the PID14 core motif is present in several plant associated fungi and bacteria, indicating the existence of a conserved microbial inhibitor of proteases (cMIP).
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.