Evidence map›Paper›PMID 30952812›Full record

ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2019

Wide-Area All-Optical Neurophysiology in Acute Brain Slices.

Samouil L Farhi, Vicente J Parot, Abhinav Grama, Masahito Yamagata, Ahmed S Abdelfattah, Yoav Adam, Shan Lou, Jeong Jun Kim, Robert E Campbell, David D Cox and 1 more

Open access · bronzeAbstract read
In one paragraph

Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.

0numbers the graph read from it
0cells of the map it votes in
32citing papers in PubMed
1.8field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

32 citing papers in PubMed, 42 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Review
  6. Optogenetic control of Nodal signaling patterns.Development (Cambridge, England) · 2025
    Article
  7. Article
  8. Article
  9. Luminos: open-source software for bidirectional microscopy.bioRxiv : the preprint server for biology · 2025
    Article
  10. Review
  11. Sensing a rainbow of colors: algal photoreceptors.Frontiers in plant science · 2025
    Review
  12. Article
  13. Article
  14. Review
  15. Optogenetic control of Nodal signaling patterns.bioRxiv : the preprint server for biology · 2024
    Article
  16. Mapping uterine calcium dynamics during the ovulatory cycle in live mice.bioRxiv : the preprint server for biology · 2024
    Article
  17. Calcium image analysis in the moving gut.Neurogastroenterology and motility · 2023
    Article
  18. Article
  19. Article
  20. TWISP: A Transgenic Worm for Interrogating Signal Propagation inbioRxiv : the preprint server for biology · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 6 institutions in 4 countries.

Samouil L FarhiChemical Biology Program.ORCID 0000-0003-1359-4568
Vicente J ParotBiophysics Program.ORCID 0000-0002-9261-6667
Abhinav GramaDepartment of Molecular and Cellular Biology.
Masahito YamagataDepartment of Molecular and Cellular Biology.ORCID 0000-0001-8193-2931
Ahmed S AbdelfattahDepartment of Chemistry, University of Alberta, Edmonton, Alberta T6G 2R3, Canada.ORCID 0000-0001-8131-1772
Yoav AdamDepartment of Chemistry and Chemical Biology.ORCID 0000-0003-2631-5417
Shan LouDepartment of Chemistry and Chemical Biology.ORCID 0000-0002-1638-9917
Jeong Jun KimDepartment of Chemistry and Chemical Biology.
Robert E CampbellDepartment of Chemistry, University of Alberta, Edmonton, Alberta T6G 2R3, Canada.ORCID 0000-0003-0604-092X
David D CoxDepartment of Molecular and Cellular Biology.
Adam E CohenDepartment of Chemistry and Chemical Biology, cohen@chemistry.harvard.edu.ORCID 0000-0002-8699-2404
Institut de Biologie Moléculaire et Cellulaire · FRUniversity of Alberta · CABiochemical Society · GBHoward Hughes Medical Institute · USMassachusetts Institute of Technology · USPaulsson (United States) · US

Funding

MOLECULAR BIOPHYSICS TRAINING GRANTT32GM008313 · NIGMS · HARVARD UNIVERSITY (MEDICAL SCHOOL) · PI MURTHY, VENKATESH N · 1989 to 2023
$14.6M
Howard Hughes Medical Institute
6 · The paper itself

Abstract

Optical tools for simultaneous perturbation and measurement of neural activity open the possibility of mapping neural function over wide areas of brain tissue. However, spectral overlap of actuators and reporters presents a challenge for their simultaneous use, and optical scattering and out-of-focus fluorescence in tissue degrade resolution. To minimize optical crosstalk, we combined an optimized variant (eTsChR) of the most blue-shifted channelrhodopsin reported to-date with a nuclear-localized red-shifted Ca

Indexed as

Action PotentialsAnimalsAnticonvulsantsExcitatory Amino Acid AntagonistsHEK293 CellsHippocampusHumansMiceNerve NetNeuronsOptical ImagingOptogeneticsPhotic StimulationRatsSynaptic TransmissionAnticonvulsantsExcitatory Amino Acid Antagonistsbrain slicecalcium imagingmicroscopyoptogenetics

Identifiers

PMID30952812
PMCPMC6670252
OpenAlexW2952367635

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.