ArticleGenetics2019
NATF (Native and Tissue-Specific Fluorescence): A Strategy for Bright, Tissue-Specific GFP Labeling of Native Proteins in
Article in Genetics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 38 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
38 citing papers in PubMed.
- Forward genetics reveals a conserved CNBHD-CNBHD interface that restrains EAG channel activation to regulate sleep.PLoS genetics · 2026Article
- Creation and manipulation of bipartite expression transgenes in C. elegans using phiC31 recombinase.Genetics · 2026Article
- Endogenous autophagosome reporters reveal redundant and non-redundant roles of LGG-1 and LGG-2 inbioRxiv : the preprint server for biology · 2026Article
- SynaptoTagMe, a toolkit for in vivo mapping and modulating neurotransmission at single-cell resolution.eLife · 2026Article
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- Intensity modulation of trichromatic split fluorescent proteins for live cell mapping.Cell reports methods · 2026Article
- ER discontinuities are common inbioRxiv : the preprint server for biology · 2026Article
- A trans-synaptic IgLON adhesion molecular complex directly contacts and clusters a nicotinic receptor.Nature communications · 2026Article
- WormSNAP: A software for fast, accurate, and unbiased detection of fluorescent puncta in C. elegans.PLoS computational biology · 2025Article
- Nicotine induces abnormal motor coupling through sensitization of a mechanosensory circuit in Caenorhabditis elegans.PLoS biology · 2025Article
- Expansion of lysosomal capacity in early adult neurons driven by TFEB/HLH-30 protects dendrite maintenance during aging in Caenorhabditis elegans.PLoS biology · 2025Article
- A modular system to label endogenous presynaptic proteins using split fluorophores in Caenorhabditis elegans.Genetics · 2025Article
- Detecting gene expression in Caenorhabditis elegans.Genetics · 2025Review
- CFI-1 functions unilaterally to restrict gap junction formation in C. elegans.Development (Cambridge, England) · 2025Article
- Neural Circuit Remodeling: Mechanistic Insights from Invertebrates.Journal of developmental biology · 2024Review
- RABR-1, an atypical Rab-related GTPase, cell-nonautonomously restricts somatosensory dendrite branching.Genetics · 2024Article
- Tissue-specific and endogenous protein labeling with split fluorescent proteins.Developmental biology · 2024Article
- UNC-30/PITX coordinates neurotransmitter identity with postsynaptic GABA receptor clustering.Development (Cambridge, England) · 2024Article
- Polarized localization of kinesin-1 and RIC-7 drives axonal mitochondria anterograde transport.The Journal of cell biology · 2024Article
- Tissue-specific and endogenous protein labeling with split fluorescent proteins.bioRxiv : the preprint server for biology · 2024Article
Corrections and comments
- Erratum issued
Authors and funding
3 authors.
Funding
Abstract
GFP labeling by genome editing can reveal the authentic location of a native protein, but is frequently hampered by weak GFP signals and broad expression across a range of tissues that may obscure cell-specific localization. To overcome these problems, we engineered a Native And Tissue-specific Fluorescence (NATF) strategy that combines genome editing and split-GFP to yield bright, cell-specific protein labeling. We use clustered regularly interspaced short palindromic repeats CRISPR/Cas9 to insert a tandem array of seven copies of the GFP11 β-strand (
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.