Evidence map›Paper›PMID 30952514›Full record

ArticleMolecular cell2019

Dynamic Recruitment of Single RNAs to Processing Bodies Depends on RNA Functionality.

Sethuramasundaram Pitchiaya, Marcio D A Mourao, Ameya P Jalihal, Lanbo Xiao, Xia Jiang, Arul M Chinnaiyan, Santiago Schnell, Nils G Walter

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular cell, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 83 papers.

0numbers the graph read from it
0cells of the map it votes in
83citing papers in PubMed
6.4field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

83 citing papers in PubMed, 132 citations in OpenAlex.

  1. Review
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  5. Divergent condensates tune transcriptional responses during stress.bioRxiv : the preprint server for biology · 2026
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  12. Perspective: An outlook on fluorescence tracking.The Journal of chemical physics · 2025
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  14. Review
  15. Review
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23 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Sethuramasundaram PitchiayaSingle Molecule Analysis Group, Department of Chemistry, University of Michigan, Ann Arbor, MI 48109-1055, USA; Michigan Center for Translational Pathology, University of Michigan Medical School, Ann Arbor, MI 48109-1055, USA; Department of Pathology, University of Michigan, Ann Arbor, MI 48109, USA; Comprehensive Cancer Center, University of Michigan, Ann Arbor, MI 48109, USA. Electronic address: sethu@umich.edu.
Marcio D A MouraoDepartment of Molecular and Integrative Physiology, University of Michigan Medical School, Ann Arbor, MI 48109-1055, USA; Consulting for Statistics, Computing and Analytics Research, University of Michigan, Ann Arbor, MI 48109-1055, USA.
Ameya P JalihalSingle Molecule Analysis Group, Department of Chemistry, University of Michigan, Ann Arbor, MI 48109-1055, USA.
Lanbo XiaoMichigan Center for Translational Pathology, University of Michigan Medical School, Ann Arbor, MI 48109-1055, USA.
Xia JiangMichigan Center for Translational Pathology, University of Michigan Medical School, Ann Arbor, MI 48109-1055, USA.
Arul M ChinnaiyanMichigan Center for Translational Pathology, University of Michigan Medical School, Ann Arbor, MI 48109-1055, USA; Department of Pathology, University of Michigan, Ann Arbor, MI 48109, USA; Comprehensive Cancer Center, University of Michigan, Ann Arbor, MI 48109, USA; Howard Hughes Medical Institute, University of Michigan, Ann Arbor, MI 48109, USA.
Santiago SchnellDepartment of Molecular and Integrative Physiology, University of Michigan Medical School, Ann Arbor, MI 48109-1055, USA.
Nils G WalterSingle Molecule Analysis Group, Department of Chemistry, University of Michigan, Ann Arbor, MI 48109-1055, USA. Electronic address: nwalter@umich.edu.
University of Michigan–Ann Arbor · USMichigan Center for Translational Pathology · USAnalysis Group (United States) · US

Funding

CELLULAR AND MOLECULAR BIOLOGY AT MICHIGANT32GM007315 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI PUTHENVEEDU, MANOJKUMAR A · 1985 to 2021
$12.8M
The RNA nanomachines of the gene expression machinery dissected at the single molecule levelR35GM131922 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI NILS G WALTER · 2019 to 2026
$6.9M
U-turn of the Hepatitis Delta Virus RibozymeR01GM062357 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI WALTER, NILS G · 2001 to 2019
$5.8M
Trekking with the Ribognome: Single Molecule Microscopy of Intracellular miRNPsR01GM081025 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI WALTER, NILS G · 2007 to 2010
$998k
NIGMS NIH HHS R01 GM062357NIGMS NIH HHS R01 GM081025NIGMS NIH HHS R35 GM131922NIGMS NIH HHS T32 GM007315
6 · The paper itself

Abstract

Cellular RNAs often colocalize with cytoplasmic, membrane-less ribonucleoprotein (RNP) granules enriched for RNA-processing enzymes, termed processing bodies (PBs). Here we track the dynamic localization of individual miRNAs, mRNAs, and long non-coding RNAs (lncRNAs) to PBs using intracellular single-molecule fluorescence microscopy. We find that unused miRNAs stably bind to PBs, whereas functional miRNAs, repressed mRNAs, and lncRNAs both transiently and stably localize within either the core or periphery of PBs, albeit to different extents. Consequently, translation potential and 3' versus 5' placement of miRNA target sites significantly affect the PB localization dynamics of mRNAs. Using computational modeling and supporting experimental approaches, we show that partitioning in the PB phase attenuates mRNA silencing, suggesting that physiological mRNA turnover occurs predominantly outside of PBs. Instead, our data support a PB role in sequestering unused miRNAs for surveillance and provide a framework for investigating the dynamic assembly of RNP granules by phase separation at single-molecule resolution.

Indexed as

Cytoplasmic GranulesGene SilencingHeLa CellsHumansMicroRNAsRibonucleoproteinsRNA, Long NoncodingRNA, MessengerRNA Processing, Post-TranscriptionalRNA, UntranslatedSingle Molecule ImagingMicroRNAsRibonucleoproteinsRNA, Long NoncodingRNA, MessengerRNA, Untranslatedlong non-coding RNAsmicroRNAsmRNAsphase separationprocessing bodiesRNP granulessingle-molecule microscopy

Identifiers

PMID30952514
PMCPMC6499680
OpenAlexW2949901525

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.