ArticleBMC complementary and alternative medicine2019
Tannin profile, antioxidant properties, and antimicrobial activity of extracts from two Mediterranean species of parasitic plant Cytinus.
Article in BMC complementary and alternative medicine, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers, 1 of them a synthesis that pooled it.
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49 citing papers in PubMed, 1 synthesis or guideline pooled it, 147 citations in OpenAlex.
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- Prevention and Treatment ofPathogens (Basel, Switzerland) · 2026Review
- Plant antimicrobials: extraction, characterization and activity against foodborne microorganisms.Folia microbiologica · 2026Review
- ExploitingAntioxidants (Basel, Switzerland) · 2026Article
- Tannins as Therapeutic Agents for Ulcerative Colitis: Mechanisms and Prospects in Regulating Gut Inflammatory-Oxidative Homeostasis.Molecules (Basel, Switzerland) · 2026Review
- Antioxidant and Health-Related Effects of Tannins: From Agri-Food By-Products to Human and Animal Health.Antioxidants (Basel, Switzerland) · 2026Review
- Phytochemical and antimicrobial properties of Morus nigra and Morus alba grown in Fujairah, United Arab Emirates.Scientific reports · 2025Article
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- Antioxidant: Antimycobacterial and Antibiofilm Activities of Acetone Extract and SubfractionAntibiotics (Basel, Switzerland) · 2024Article
- A Comprehensive Analysis of Diversity, Structure, Biosynthesis and Extraction of Biologically Active Tannins from Various Plant-Based Materials Using Deep Eutectic Solvents.Molecules (Basel, Switzerland) · 2024Review
- Plant-Based Films and Hydrogels for Wound Healing.Microorganisms · 2024Review
- Use of Essential Oils to Counteract the Phenomena of Antimicrobial Resistance in Livestock Species.Antibiotics (Basel, Switzerland) · 2024Review
- Proteomic study of the inhibitory effects of tannic acid on MRSA biofilm.Frontiers in pharmacology · 2024Article
- Inhibitory effect of natural compounds on quorum sensing system inFrontiers in pharmacology · 2024Review
- Effects ofMicroorganisms · 2023Article
- Mediterranean Plants with Antimicrobial Activity againstMicroorganisms · 2023Article
- Phytochemical Screening on Phenolic, Flavonoid Contents, and Antioxidant Activities of Six Indigenous Plants Used in Traditional Thai Medicine.International journal of molecular sciences · 2023Article
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- Antibacterial and Antibiofilm Effects of Lactobacilli Strains against Clinical Isolates ofAntibiotics (Basel, Switzerland) · 2023Article
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6 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundCytinus is small genus of endophytic parasitic plants distributed in South Africa, Madagascar, and in the Mediterranean region. In the latter area, two species occur, Cytinus hypocistis and C. ruber, distinguished by both morphological characters and ecological traits. We characterized the ethanolic and aqueous extracts obtained from the inflorescences of C. hypocistis and C. ruber collected in Sardinia, Italy, and explored their tannin content, antioxidant properties and antimicrobial activities.
methodsTotal phenolic contents were determined by Folin-Ciocalteu spectrophotometric method. Tannin content was determined by HPLC. Antioxidant activity of the extracts was tested with both electron transfer-based (FRAP, TEAC, DPPH) and spectrophotometric HAT methods (ORAC-PYR). The antimicrobial activities of extracts/compounds were evaluated using the broth microdilution method. The bactericidal activity was evaluated using the time-kill method. Biofilm formation was evaluated by crystal violet (CV) staining assay.
resultsCharacterization of the tannin profile of C. hypocistis and C. ruber revealed a significant amount of gallotannins, in particular 1-O-galloyl-β-D-glucose. In addition, pentagalloyl-O-β-D-glucose was present in all extracts, reaching the concentration of 0.117 g/kg in the ethanolic extract of C. hypocistis. C. hypocistis extracts displayed a strongest antioxidant activity than C. ruber extracts. Three Gram-positive bacterial species tested (Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecium) resulted sensitive to both Cytinus extracts, with MICs ranging from 125 to 500 μg/ml for aqueous extracts and from 31.25 to 250 μg/ml for ethanolic extracts; on the contrary, Gram-negative strains (Pseudomonas aeruginosa and Klebsiella pneumoniae) were not affected by Cytinus extracts. Intriguingly, we observed the suppressive activity of ethanolic extracts of C. hypocistis and C. ruber on biofilm formation of S. epidermidis. Experiments performed with synthetic compounds indicated that pentagalloyl-O-β-D-glucose is likely to be one of the active antimicrobial components of Cytinus extracts.
conclusionsThese findings show that Cytinus extracts have antimicrobial and antioxidant activities, suggesting a possible application of Cytinus as sources of natural antimicrobials and antioxidants.
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