ArticlePloS one2019
Evaluation of the comparative accuracy of the complement fixation test, Western blot and five enzyme-linked immunosorbent assays for serodiagnosis of glanders.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 49 citations in OpenAlex.
- Glanders: Past, Present and Future of a Neglected Zoonosis as a Threat in Veterinary and Human Health.Veterinary medicine and science · 2026Review
- Glanders: Historical military use and potential bioterrorism concern.Open veterinary journal · 2025Review
- Isolation and Purification of theArchives of Razi Institute · 2025Article
- Comparison of immunogenicity of 17 Burkholderia mallei antigens and whole cell lysate using indirect ELISA.The Journal of veterinary medical science · 2025Article
- Reassessing the distribution of Burkholderia pseudomallei outside known endemic areas using animal serological screening combined with environmental surveys: The case of Les Saintes (Guadeloupe) and French Guiana.PLoS neglected tropical diseases · 2024Article
- Isolation of Burkholderia pseudomallei from a goat in New Caledonia: implications for animal and human health monitoring and serological tool comparison.BMC veterinary research · 2024Article
- Development and validation of a chemiluminescent western blot assay for glanders (Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2024Article
- Biodistribution of RNA Vaccines and of Their Products: Evidence from Human and Animal Studies.Biomedicines · 2023Review
- Epidemiological Situation of Glanders in the State of Pará, Brazil.Pathogens (Basel, Switzerland) · 2023Article
- Protein Microarray-Guided Development of a Highly Sensitive and Specific Dipstick Assay for Glanders Serodiagnostics.Journal of clinical microbiology · 2023Article
- Influence of genomic variations on glanders serodiagnostic antigens using integrative genomic and transcriptomic approaches.Frontiers in veterinary science · 2023Article
- Silent Antibodies Start Talking: Enhanced Lateral Flow Serodiagnosis with Two-Stage Incorporation of Labels into Immune Complexes.Biosensors · 2022Article
- First glanders cases detected in Nepal underscore the need for surveillance and border controls.BMC veterinary research · 2022Article
- Genetic diversity and spatial distribution of Burkholderia mallei by core genome-based multilocus sequence typing analysis.PloS one · 2022Article
- A glycoengineered antigen exploiting a conserved protein O-glycosylation pathway in the Burkholderia genus for detection of glanders infections.Virulence · 2021Article
- Validation of a Commercial Glanders ELISA as an Alternative to the CFT in International Trade of Equidae.Frontiers in veterinary science · 2021Article
- Systematic monitoring of glanders-infected horses by complement fixation test, bacterial isolation, and PCR.Veterinary and animal science · 2020Article
- Seroprevalence of equine glanders in horses in the central and eastern parts of Mongolia.The Journal of veterinary medical science · 2020Article
Corrections and comments
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Authors and funding
14 authors at 8 institutions in 6 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Glanders is a zoonotic contagious disease of equids caused by Burkholderia (B.) mallei. Serodiagnosis of the disease is challenging because of false-positive and false-negative test results. The accuracy of the complement fixation test (CFT) which is prescribed for international trade by the World Organisation for Animal Health (OIE), five ELISAs and a Western blot (WB) were compared for serodiagnosis of glanders using sera from 3,000 glanders-free and 254 glanderous equids. Four ELISA tests are based on recombinant antigens (TssA, TssB, BimA and Hcp1), the IDVet ELISA is based on a semi-purified fraction of B. mallei and WB makes use of a purified LPS-containing B. mallei-antigen. Sensitivity and specificity of tests were estimated using cut-off values recommended by the test developers. The WB and all ELISAs, except BimA, were significantly more specific than the CFT. ELISAs based on TssA, TssB, and BimA antigens had significantly lower sensitivity compared to CFT while the sensitivities of the Hcp1-ELISA, the IDVet-ELISA and the WB did not differ significantly from that of the CFT. Given their comparable sensitivities and specificities, the CFT (98.0%, 96.4%), the WB (96.8%, 99.4%), the Hcp1-ELISA (95.3%, 99.6%) and the IDVet-ELISA (92.5%, 99.5%) should be further developed to meet OIE requirements.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.