ArticleRNA biology2019
Structural features of an Xrn1-resistant plant virus RNA.
Article in RNA biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 26 citations in OpenAlex.
- Advances in Plant Antiviral RNAi: From Host DCLs/RDRs to Diversified Viral Counteracting Strategies.Viruses · 2026Review
- The myriad roles of RNA structure in the flavivirus life cycle.RNA biology · 2024Review
- Xrn1-resistant RNA motifs are disseminated throughout the RNA virome and are able to block scanning ribosomes.Scientific reports · 2023Article
- Investigating the correlation between Xrn1-resistant RNAs and frameshifter pseudoknots.RNA biology · 2023Article
- Replication of alphaviruses requires a pseudoknot that involves the poly(A) tail.RNA (New York, N.Y.) · 2022Article
- Effects of the Noncoding Subgenomic RNA of Red Clover Necrotic Mosaic Virus in Virus Infection.Journal of virology · 2022Article
- A plant-infecting subviral RNA associated with poleroviruses produces a subgenomic RNA which resists exonuclease XRN1 in vitro.Virology · 2022Article
- Evolutionary traits of Tick-borne encephalitis virus: Pervasive non-coding RNA structure conservation and molecular epidemiology.Virus evolution · 2022Article
- Mechanical strength of RNA knot in Zika virus protects against cellular defenses.Nature chemical biology · 2021Article
- Replication-Dependent Biogenesis of Turnip Crinkle Virus Long Noncoding RNAs.Journal of virology · 2021Article
- Article
- Structural Analysis and Whole Genome Mapping of a New Type of Plant Virus Subviral RNA: Umbravirus-Like Associated RNAs.Viruses · 2021Article
- Shared properties and singularities of exoribonuclease-resistant RNAs in viruses.Computational and structural biotechnology journal · 2021Review
- Discoveries of Exoribonuclease-Resistant Structures of Insect-Specific Flaviviruses Isolated in Zambia.Viruses · 2020Article
- Biochemical Characterization of Yeast Xrn1.Biochemistry · 2020Article
- A 212-nt long RNA structure in the Tobacco necrosis virus-D RNA genome is resistant to Xrn degradation.Nucleic acids research · 2019Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Xrn1 is a major 5'-3' exoribonuclease involved in the RNA metabolism of many eukaryotic species. RNA viruses have evolved ways to thwart Xrn1 in order to produce subgenomic non-coding RNA that affects the hosts RNA metabolism. The 3' untranslated region of several beny- and cucumovirus RNAs harbors a so-called 'coremin' motif that is required for Xrn1 stalling. The structural features of this motif have not been studied in detail yet. Here, by using in vitro Xrn1 degradation assays, we tested over 50 different RNA constructs based on the Beet necrotic yellow vein virus sequence to deduce putative structural features responsible for Xrn1 stalling. We demonstrated that the minimal benyvirus stalling site consists of two hairpins of 3 and 4 base pairs respectively. The 5' proximal hairpin requires a YGAD (Y = U/C, D = G/A/U) consensus loop sequence, whereas the 3' proximal hairpin loop sequence is variable. The sequence of the 10-nucleotide spacer that separates the hairpins is highly conserved and potentially involved in tertiary interactions. Similar coremin motifs were identified in plant virus isolates from other families including Betaflexiviridae, Virgaviridae, Potyviridae and Secoviridae (order of the Picornavirales). We conclude that Xrn1-stalling motifs are more widespread among RNA viruses than previously realized.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.