Evidence map›Paper›PMID 30950791›Full record

ArticleeLife2019

Decoys provide a scalable platform for the identification of plant E3 ubiquitin ligases that regulate circadian function.

Ann Feke, Wei Liu, Jing Hong, Man-Wah Li, Chin-Mei Lee, Elton K Zhou, Joshua M Gendron

Open access · goldAbstract read
In one paragraph

Article in eLife, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.

0numbers the graph read from it
0cells of the map it votes in
23citing papers in PubMed
6.4field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

23 citing papers in PubMed, 42 citations in OpenAlex.

  1. Article
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  9. A circadian clock output functions independently of phyB to sustain daytime PIF3 degradation.Proceedings of the National Academy of Sciences of the United States of America · 2024
    Article
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  14. Mutations that alter Arabidopsis flavonoid metabolism affect the circadian clock.The Plant journal : for cell and molecular biology · 2022
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 2 countries.

Ann FekeDepartment of Molecular, Cellular and Developmental Biology, Yale University, New Haven, United States.ORCID 0000-0002-8246-0056
Wei Liu *Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, United States.
Jing Hong *Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, United States.
Man-Wah LiDepartment of Molecular, Cellular and Developmental Biology, Yale University, New Haven, United States.
Chin-Mei LeeDepartment of Molecular, Cellular and Developmental Biology, Yale University, New Haven, United States.ORCID 0000-0003-3870-4268
Elton K ZhouDepartment of Molecular, Cellular and Developmental Biology, Yale University, New Haven, United States.
Joshua M GendronDepartment of Molecular, Cellular and Developmental Biology, Yale University, New Haven, United States.ORCID 0000-0001-8605-3047
Yale University · US

Funding

Yale Clinical and Translational Science Award (U Component)UL1TR001863 · NCATS · YALE UNIVERSITY · PI John H. Krystal, LUCILA OHNO-MACHADO · 2016 to 2026
$102.9M
PREDOCTORAL TRAINING PROGRAM IN GENETICST32GM007499 · NIGMS · YALE UNIVERSITY · PI CARLSON, JOHN R, REINKE, VALERIE J · 1985 to 2022
$13.9M
Protein degradation mechanisms that regulate daily and seasonal timingR35GM128670 · NIGMS · YALE UNIVERSITY · PI Joshua Martin Gendron · 2018 to 2026
$4.0M
National Science Foundation EAGER #1548538National Science Foundation GRFP DGE-1122492NCATS NIH HHS UL1 TR001863NIGMS NIH HHS R35 GM128670NIGMS NIH HHS T32 GM007499NIH HHS R35 GM128670NIH HHS T32 GM007499
6 · The paper itself

Abstract

The circadian clock relies on regulated degradation of clock proteins to maintain rhythmicity. Despite this, we know few components that mediate protein degradation. This is due to high levels of functional redundancy within plant E3 ubiquitin ligase families. In order to overcome this issue and discover E3 ubiquitin ligases that control circadian function, we generated a library of transgenic Arabidopsis plants expressing dominant-negative 'decoy' E3 ubiquitin ligases. We determined their effects on the circadian clock and identified dozens of new potential regulators of circadian function. To demonstrate the potency of the decoy screening methodology to overcome redundancy and identify

Indexed as

Circadian ClocksGene Expression Regulation, PlantArabidopsisArabidopsis ProteinsCircadian Rhythm Signaling Peptides and ProteinsGenetic TestingMutant ProteinsPlants, Genetically ModifiedProteolysisRecombinant ProteinsUbiquitin-Protein LigasesArabidopsis ProteinsCircadian Rhythm Signaling Peptides and ProteinsMutant ProteinsRecombinant ProteinsUbiquitin-Protein LigasesA. thalianacircadian clockE3 ubiquitin ligase decoyplant biologypost-translational modificationprotein ubiquitylation

Identifiers

PMID30950791
PMCPMC6483598
OpenAlexW2934537932

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.