Trans Complementation of Replication-defective Omsk Hemorrhagic Fever Virus for Antiviral Study.

Qiuyan Zhang, Na Li, Chenglin Deng, Zherui Zhang, Xiaodan Li, Kentaro Yoshii, Hanqing Ye, Bo Zhang

Abstract read

In one paragraph

Article in Virologica Sinica, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it

0cells of the map it votes in

10citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed.

A replication-defective tick-borne encephalitis virus generated by NS1 trans-complementation confers robust protective efficacy.Virologica Sinica · 2026
Article
Fluorescent Powassan Reporter Viruses Infect Neuron, Astrocyte and Microglial Cell Lines Independent of Attenuating D308N Envelope Protein Modification.Viruses · 2026
Article
Standardized Diagnostic Assays for Omsk Hemorrhagic Fever Virus.Pathogens (Basel, Switzerland) · 2025
Article
Omsk hemorrhagic fever virus: review of the biology, ecology, and disease associated with a historic tick-borne pathogen.Infectious diseases & immunity · 2025
Review
Targeting Polyprotein to Design Potential Multiepitope Vaccine againstBiology · 2024
Article
Geographical distribution and pathogenesis of ticks and tick-borne viral diseases.Frontiers in microbiology · 2023
Review
Generation and Application of a Luciferase Reporter Virus Based on Yellow Fever Virus 17D.Virologica Sinica · 2021
Article
Development and Characterization of SYBR Green I Based RT-PCR Assay for Detection of Omsk Hemorrhagic Fever Virus.Virologica Sinica · 2021
Article
Application of a Biologically Contained Reporter System To Study Gain-of-Function H5N1 Influenza A Viruses with Pandemic Potential.mSphere · 2020
Article
A Sensitive Yellow Fever Virus Entry Reporter Identifies Valosin-Containing Protein (VCP/p97) as an Essential Host Factor for Flavivirus Uncoating.mBio · 2020
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Qiuyan ZhangKey Laboratory of Special Pathogens and Biosafety, Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China.

Na LiKey Laboratory of Special Pathogens and Biosafety, Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China.

Chenglin DengKey Laboratory of Special Pathogens and Biosafety, Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China.

Zherui ZhangKey Laboratory of Special Pathogens and Biosafety, Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China.

Xiaodan LiKey Laboratory of Special Pathogens and Biosafety, Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China.

Kentaro YoshiiLaboratory of Public Health, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, 060-0818, Japan.

Hanqing YeKey Laboratory of Special Pathogens and Biosafety, Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China.

Bo ZhangKey Laboratory of Special Pathogens and Biosafety, Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China. zhangbo@wh.iov.cn.ORCID http://orcid.org/0000-0002-8895-3679

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Omsk hemorrhagic fever virus (OHFV) is a tick-borne flavivirus classified as a biosafety level-4 (BSL4) pathogen. Studies of OHFV are restricted to be conducted within BSL4 laboratories. Currently, no commercial vaccines or antiviral drugs are available against OHFV infection. In this study, we recovered a replication-deficient OHFV with an NS1 deletion (OHFV-ΔNS1) and reporter virus replacing NS1 with the Gaussia luciferase (Gluc) (OHFV-ΔNS1-Gluc). Both the defective OHFV-ΔNS1 and OHFV-ΔNS1-Gluc virus could only replicate efficiently in the BHK21 cell line expressing NS1 (BHK21

Indexed as

Virus ReplicationAnimalsAntiviral AgentsCell LineCricetinaeEncephalitis Viruses, Tick-BorneGenetic Complementation TestLuciferasesViral Nonstructural ProteinsAntiviral AgentsLuciferasesViral Nonstructural ProteinsAntiviral screeningGaussia luciferase (Gluc)NS1Omsk hemorrhagic fever virus (OHFV)Trans complementation

Identifiers

PMID30949960

DOI10.1007/s12250-019-00109-0

PMCPMC6687815

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.