Evidence map›Paper›PMID 30949841›Full record

ArticlePharmaceutical research2019

Identification of Lysine Misincorporation at Asparagine Position in Recombinant Insulin Analogs Produced in E. coli.

Dorota Stadnik, Anna Bierczyńska-Krzysik, Joanna Zielińska, Jarosław Antosik, Piotr Borowicz, Elżbieta Bednarek, Wojciech Bocian, Jerzy Sitkowski, Lech Kozerski

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Pharmaceutical research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
2.7field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 12 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Dorota StadnikŁukasiewicz Research Network - Institute of Biotechnology and Antibiotics, Starościńska 5, 02-516, Warsaw, Poland. stadnikd@iba.waw.pl.ORCID http://orcid.org/0000-0003-2864-672X
Anna Bierczyńska-KrzysikŁukasiewicz Research Network - Institute of Biotechnology and Antibiotics, Starościńska 5, 02-516, Warsaw, Poland.
Joanna ZielińskaŁukasiewicz Research Network - Institute of Biotechnology and Antibiotics, Starościńska 5, 02-516, Warsaw, Poland.
Jarosław AntosikŁukasiewicz Research Network - Institute of Biotechnology and Antibiotics, Starościńska 5, 02-516, Warsaw, Poland.
Piotr BorowiczŁukasiewicz Research Network - Institute of Biotechnology and Antibiotics, Starościńska 5, 02-516, Warsaw, Poland.
Elżbieta BednarekNational Medicines Institute, Chełmska 30/34, 00-725, Warsaw, Poland.
Wojciech BocianNational Medicines Institute, Chełmska 30/34, 00-725, Warsaw, Poland.
Jerzy SitkowskiNational Medicines Institute, Chełmska 30/34, 00-725, Warsaw, Poland.
Lech KozerskiNational Medicines Institute, Chełmska 30/34, 00-725, Warsaw, Poland.
Instytut Biotechnologii i Antybiotyków · PLNarodowy Instytut Leków · PL

Funding

European Regional Development Fund POIG. 01.01.02-00-007/08-04Narodowe Centrum Badań i Rozwoju PBS2/A9/27/2013
6 · The paper itself

Abstract

purposeIdentification of human insulin analogs' impurity with a mass shift +14 Da in comparison to a parent protein.

methodsThe protein sequence variant was detected and identified with the application of peptide mapping, liquid chromatography, tandem mass spectrometric analysis, nuclear magnetic resonance spectroscopy (NMR) and Edman sequencing.

resultsThe misincorporated lysine (Lys) at asparagine (Asn) position A21 was detected in recombinant human insulin and its analogs.

conclusionsAlthough there are three asparagine residues in the insulin derivative, the misincorporation of lysine occurred only at position A21. The process involves G/U or A/U wobble base pairing.

Indexed as

AsparagineChromatography, High Pressure LiquidEscherichia coliHumansInsulinsLysinePeptidesRecombinant ProteinsTandem Mass SpectrometryAsparagineInsulinsLysinePeptidesRecombinant Proteinsinsulin analogsinsulin impuritiesmass spectrometrymisincorporationNMR

Identifiers

PMID30949841
PMCPMC6449291
OpenAlexW2928383447

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.