Evidence map›Paper›PMID 30949775›Full record

ArticleExtremophiles : life under extreme conditions2019

High-quality draft genome sequence of Pseudomonas aeruginosa san ai, an environmental isolate resistant to heavy metals.

Lidija Izrael-Živković, Vladimir Beškoski, Milena Rikalović, Snježana Kazazić, Nicole Shapiro, Tanja Woyke, Gordana Gojgić-Cvijović, Miroslav M Vrvić, Nela Maksimović, Ivanka Karadžić

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Article in Extremophiles : life under extreme conditions, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.8field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 12 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 2 countries.

Lidija Izrael-ŽivkovićDepartment of Chemistry, Faculty of Medicine, University of Belgrade, Belgrade, Serbia.
Vladimir BeškoskiFaculty of Chemistry, University of Belgrade, Belgrade, Serbia.
Milena RikalovićFaculty of Applied Ecology Futura, Singidunum University, Belgrade, Serbia.
Snježana KazazićRuđerBošković Institute, Zagreb, Croatia.
Nicole ShapiroDOE Joint Genome Institute, Walnut Creek, CA, USA.
Tanja WoykeDOE Joint Genome Institute, Walnut Creek, CA, USA.
Gordana Gojgić-CvijovićDepartment of Chemistry, Institute of Chemistry, Technology and Metallurgy, University of Belgrade, Belgrade, Serbia.
Miroslav M VrvićFaculty of Chemistry, University of Belgrade, Belgrade, Serbia.
Nela MaksimovićDepartment of Human Genetics, Faculty of Medicine, University of Belgrade, Belgrade, Serbia.
Ivanka KaradžićDepartment of Chemistry, Faculty of Medicine, University of Belgrade, Belgrade, Serbia. ivanka.karadzic@med.bg.ac.rs.
University of Belgrade · RSJoint Genome Institute · USSingidunum University · RS

Funding

Ministry of Science of Republic of Serbia III43004Office of Science of the U.S. Department of Energy Contract No. DE-AC02-05CH11231
6 · The paper itself

Abstract

The strain Pseudomonas aeruginosa san ai, isolated from an extreme environment (industrial mineral cutting oil, pH 10), is able to survive and persist in the presence of a variety of pollutants such as heavy metals and organic chemicals. The genome of P. aeruginosa san ai is 6.98 Mbp long with a GC content of 66.08% and 6485 protein encoding genes. A large number of genes associated with proteins, responsible for microbial resistance to heavy metal ions and involved in catabolism of toxic aromatic organic compounds were identified. P. aeruginosa san ai is a highly cadmium-resistant strain. Proteome analysis of biomass after cadmium exposal confirmed a high tolerance to sublethal concentrations of cadmium (100 mg/L), based on: extracellular biosorption, bioaccumulation, biofilm formation, controlled siderophore production and a pronounced metalloprotein synthesis. Proteins responsible for survival in osmostress conditions during exposure to elevated concentrations of cadmium (200 mg/L) demonstrate a strong genetic potential of P. aeruginosa san ai for survival and adaptation. Sequencing of P. aeruginosa san ai genome provides valuable insights into the evolution and adaptation of this microbe to environmental extremes at the whole-genome level, as well as how to optimally use the strain in bioremediation of chemically polluted sites.

Indexed as

Genome, BacterialCadmiumDrug Resistance, BacterialEnvironmental PollutionMolecular Sequence AnnotationProteomePseudomonas aeruginosaCadmiumProteomeChemical pollutionCleaning polluted areasEnvironmental isolateGenomePseudomonas aeruginosa

Identifiers

PMID30949775
OpenAlexW2930726348

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.