ArticleNature communications2019
Miniaturised interaction proteomics on a microfluidic platform with ultra-low input requirements.
Article in Nature communications, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 38 citations in OpenAlex.
- Mechanisms and Research Methods of Protein Modification in Virus Entry.Applied biochemistry and biotechnology · 2025Review
- Development of a Thiol-ene Microfluidic Chip for Hydrogen/Deuterium Exchange Mass Spectrometry (HDX-MS).Analytical chemistry · 2025Article
- Micropillar arrays, wide window acquisition and AI-based data analysis improve comprehensiveness in multiple proteomic applications.Nature communications · 2024Article
- Immunopeptidomics in the Era of Single-Cell Proteomics.Biology · 2023Article
- High-Efficiency Enrichment by Saturating Nanoliters of Protein Affinity Media.Analytical chemistry · 2023Article
- Unlocking the potential of microfluidics in mass spectrometry-based immunopeptidomics for tumor antigen discovery.Cell reports methods · 2023Review
- Fused Deposition Modeling of Microfluidic Chips in Transparent Polystyrene.Micromachines · 2021Article
- Article
- Off-the-shelf proximity biotinylation for interaction proteomics.Nature communications · 2021Article
- Micro-Droplet Platform for Exploring the Mechanism of Mixed Field Agglutination in BBiosensors · 2021Article
- Microfluidics for Peptidomics, Proteomics, and Cell Analysis.Nanomaterials (Basel, Switzerland) · 2021Review
- A Toolbox for Efficient Proximity-Dependent Biotinylation in Zebrafish Embryos.Molecular & cellular proteomics : MCP · 2021Article
- Purification and enrichment of specific chromatin loci.Nature methods · 2020Review
- Protein and Proteome Measurements with Microfluidic Chips.Analytical chemistry · 2020Review
- Classification of mouse B cell types using surfaceome proteotype maps.Nature communications · 2019Article
- Mapping protein-protein interactions by mass spectrometry.Mass spectrometry reviewsReview
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Essentially all cellular processes are orchestrated by protein-protein interactions (PPIs). In recent years, affinity purification coupled to mass spectrometry (AP-MS) has been the preferred method to identify cellular PPIs. Here we present a microfluidic-based AP-MS workflow, called on-chip AP-MS, to identify PPIs using minute amounts of input material. By using this automated platform we purify the human Cohesin, CCC and Mediator complexes from as little as 4 micrograms of input lysate, representing a 50─100-fold downscaling compared to regular microcentrifuge tube-based protocols. We show that our platform can be used to affinity purify tagged baits as well as native cellular proteins and their interaction partners. As such, our method holds great promise for future biological and clinical AP-MS applications in which sample amounts are limited.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.