ArticleProceedings of the National Academy of Sciences of the United States of America2019
Rational conversion of chromophore selectivity of cyanobacteriochromes to accept mammalian intrinsic biliverdin.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 57 citations in OpenAlex.
- An ultralow-background far-red light-responsive optogenetic tool based on an engineered biliverdin-binding domain.Nature methods · 2026Article
- Mechanisms of Red-Shifted Absorption in Biliverdin-Binding Proteins.Biochemistry · 2026Article
- Discovery and Engineering of Unique Cyanobacteriochrome GAF Domains.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Rotameric Heterogeneity of Conserved Tryptophan Is Responsible for Reduced Photochemical Quantum Yield in Cyanobacteriochrome Slr1393g3.Chemphyschem : a European journal of chemical physics and physical chemistry · 2025Article
- Intracellular biliverdin dynamics during ferroptosis.Journal of biochemistry · 2024Article
- Cyanobacteriochromes: A Rainbow of Photoreceptors.Annual review of microbiology · 2024Review
- High Fluorescence of Phytochromes Does Not Require Chromophore Protonation.Molecules (Basel, Switzerland) · 2024Article
- Red/green cyanobacteriochromes acquire isomerization from phycocyanobilin to phycoviolobilin.Protein science : a publication of the Protein Society · 2024Article
- Photocobilins integrate BNature communications · 2024Article
- Cyanobacteriochromes from Gloeobacterales Provide New Insight into the Diversification of Cyanobacterial Photoreceptors.Journal of molecular biology · 2024Article
- A Single-Site Mutation Tunes Fluorescence and Chromophorylation of an Orange Fluorescent Cyanobacteriochrome.Chembiochem : a European journal of chemical biology · 2023Article
- Zinc-Induced Fluorescence Turn-On in Native and Mutant Phycoerythrobilin-Binding Orange Fluorescent Proteins.Biochemistry · 2023Article
- A Single Site Mutation Tunes Fluorescence and Chromophorylation of an Orange Fluorescent Cyanobacteriochrome.bioRxiv : the preprint server for biology · 2023Article
- Engineering of bidirectional, cyanobacteriochrome-based light-inducible dimers (BICYCL)s.Nature methods · 2023Article
- Novel cyanobacteriochrome photoreceptor with the second Cys residue showing atypical orange/blue reversible photoconversion.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2023Article
- Light- and pH-dependent structural changes in cyanobacteriochrome AnPixJg2.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2022Article
- Protein-chromophore interactions controlling photoisomerization in red/green cyanobacteriochromes.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2022Article
- Biliverdin incorporation into the cyanobacteriochrome SPI1085g3 fromFrontiers in microbiology · 2022Article
- A variety of photoreceptors and the frontiers of optogenetics.Biophysics and physicobiology · 2022Article
- The Red Edge: Bilin-Binding Photoreceptors as Optogenetic Tools and Fluorescence Reporters.Chemical reviews · 2021Review
Corrections and comments
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Authors and funding
15 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Because cyanobacteriochrome photoreceptors need only a single compact domain for chromophore incorporation and for absorption of visible spectra including the long-wavelength far-red region, these molecules have been paid much attention for application to bioimaging and optogenetics. Most cyanobacteriochromes, however, have a drawback to incorporate phycocyanobilin that is not available in the mammalian cells. In this study, we focused on biliverdin (BV) that is a mammalian intrinsic chromophore and absorbs the far-red region and revealed that replacement of only four residues was enough for conversion from BV-rejective cyanobacteriochromes into BV-acceptable molecules. We succeeded in determining the crystal structure of one of such engineered molecules, AnPixJg2_BV4, at 1.6 Å resolution. This structure identified unusual covalent bond linkage, which resulted in deep BV insertion into the protein pocket. The four mutated residues contributed to reducing steric hindrances derived from the deeper insertion. We introduced these residues into other domains, and one of them, NpF2164g5_BV4, produced bright near-infrared fluorescence from mammalian liver in vivo. Collectively, this study provides not only molecular basis to incorporate BV by the cyanobacteriochromes but also rational strategy to open the door for application of cyanobacteriochromes to visualization and regulation of deep mammalian tissues.
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