ArticlePloS one2019
Benchmarking miniaturized microscopy against two-photon calcium imaging using single-cell orientation tuning in mouse visual cortex.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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Who cites it
15 citing papers in PubMed, 28 citations in OpenAlex.
- Open-Source Platform for Adjustable Training Regimes in Freely Moving and Head-Fixed Mice.eNeuro · 2026Article
- CaBLAM: a high-contrast bioluminescent CaNature methods · 2026Article
- CaBLAM! A high-contrast bioluminescent CabioRxiv : the preprint server for biology · 2025Article
- Review
- Advances in cellular resolution microscopy for brain imaging in rats.Neurophotonics · 2023Review
- Anteromedial Thalamus Gates the Selection & Stabilization of Long-Term Memories.bioRxiv : the preprint server for biology · 2023Article
- Emerging approaches for decoding neuropeptide transmission.Trends in neurosciences · 2022Review
- Article
- Calcium imaging: A versatile tool to examine Huntington's disease mechanisms and progression.Frontiers in neuroscience · 2022Review
- Application of optogenetics and in vivo imaging approaches for elucidating the neurobiology of addiction.Molecular psychiatry · 2022Review
- Mouse visual cortex areas represent perceptual and semantic features of learned visual categories.Nature neuroscience · 2021Article
- Diving into the brain: deep-brain imaging techniques in conscious animals.The Journal of endocrinology · 2020Review
- Design of a high-resolution light field miniscope for volumetric imaging in scattering tissue.Biomedical optics express · 2020Article
- Imaging spinal cord activity in behaving animals.Experimental neurology · 2019Review
- Circuit Investigations With Open-Source Miniaturized Microscopes: Past, Present and Future.Frontiers in cellular neuroscience · 2019Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Miniaturized microscopes are lightweight imaging devices that allow optical recordings from neurons in freely moving animals over the course of weeks. Despite their ubiquitous use, individual neuronal responses measured with these microscopes have not been directly compared to those obtained with established in vivo imaging techniques such as bench-top two-photon microscopes. To achieve this, we performed calcium imaging in mouse primary visual cortex while presenting animals with drifting gratings. We identified the same neurons in image stacks acquired with both microscopy methods and quantified orientation tuning of individual neurons. The response amplitude and signal-to-noise ratio of calcium transients recorded upon visual stimulation were highly correlated between both microscopy methods, although influenced by neuropil contamination in miniaturized microscopy. Tuning properties, calculated for individual orientation tuned neurons, were strongly correlated between imaging techniques. Thus, neuronal tuning features measured with a miniaturized microscope are quantitatively similar to those obtained with a two-photon microscope.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.