ArticleMicrobial cell factories2019
Construction of DGLA producing cell factory by genetic modification of Mucor circinelloides.
Article in Microbial cell factories, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Transcriptomic Analysis of Lipid Accumulation in Mucor circinelloides WJ11 Under Submerged and Solid-State Fermentation.Applied biochemistry and biotechnology · 2025Article
- Recent advances and future directions on GLA-producing organisms.Frontiers in bioengineering and biotechnology · 2025Review
- Genetic Manipulation in Mucorales and New Developments to Study Mucormycosis.International journal of molecular sciences · 2022Review
- Mucor circinelloides: a model organism for oleaginous fungi and its potential applications in bioactive lipid production.Microbial cell factories · 2022Review
- Microbes: A Hidden Treasure of Polyunsaturated Fatty Acids.Frontiers in nutrition · 2022Review
- Simultaneous overexpression of ∆6-, ∆12- and ∆9-desaturases enhanced the production of γ-linolenic acid inFrontiers in microbiology · 2022Article
- Evaluation of Different Standard Amino Acids to Enhance the Biomass, Lipid, Fatty Acid, and γ-Linolenic Acid Production inFrontiers in nutrition · 2022Article
- Recent Molecular Tools for the Genetic Manipulation of Highly Industrially Important Mucoromycota Fungi.Journal of fungi (Basel, Switzerland) · 2021Review
- Mass spore production of3 Biotech · 2021Article
- Role of Snf-β in lipid accumulation in the high lipid-producing fungus Mucor circinelloides WJ11.Microbial cell factories · 2021Article
- Genetic Modification ofFrontiers in nutrition · 2021Article
- The Potential of Single-Cell Oils Derived From Filamentous Fungi as Alternative Feedstock Sources for Biodiesel Production.Frontiers in microbiology · 2021Review
- Increased Accumulation of Medium-Chain Fatty Acids by Dynamic Degradation of Long-Chain Fatty Acids inGenes · 2020Article
- Comparative genomics applied to Mucor species with different lifestyles.BMC genomics · 2020Article
- Mitochondrial Citrate Transport System in the Fungus Mucor circinelloides: Identification, Phylogenetic Analysis, and Expression Profiling During Growth and Lipid Accumulation.Current microbiology · 2020Article
- Comparative Analysis of β-Carotene Production byMetabolites · 2020Article
- Physiological Traits of Dihomo-γ-Linolenic Acid Production of the EngineeredFrontiers in microbiology · 2020Article
- Article
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7 authors.
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Abstract
backgroundDihomo-gamma linolenic acid (DGLA, 20:3, n-6) is the elongated product of Gamma linolenic acid (GLA, 18:3, n-6) catalyzed by the enzyme delta-6 elongase (D6E) or gamma linolenic acid elongase (GLELO). Construction of engineered oleaginous microbes have been attracting significant interest to produce DGLA because of its nutritional value and medicinal applications. Mucor circinelloides is a GLA producing filamentous fungus which can be a useful tool to produce DGLA. We have, therefore, overexpressed the D6E (GLELO) gene in this fungus to construct DGLA producing cell factory.
resultTo produce DGLA in M. circinelloides, homologous overexpression of D6E (GLELO) gene was analyzed. When the gene was overexpressed in M. circinelloides CBS277.49, up to 5.72% DGLA was produced in this strain.
conclusionTo our knowledge, this is the first report describing the overexpression of D6E (GLELO) gene in M. circinelloides to construct DGLA producing cell factory. A new scope for further research has been established by this work for improved production of DGLA in this fungus, specifically in its high lipid-producing strain, WJ11.
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