ArticlePLoS neglected tropical diseases2019
Febrile temperatures increase in vitro antibody affinity for malarial and dengue antigens.
Article in PLoS neglected tropical diseases, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Overview of antibody-drug conjugates nonclinical and clinical toxicities and related contributing factors.Antibody therapeutics · 2025Review
- Integrating thermodynamics and molecular kinetics into western blotting teaching.Frontiers in molecular biosciences · 2025Article
- Plasmodium Falciparum and mosquito vector IgG patterns across suspected malaria cases in Ghana.BMC infectious diseases · 2024Article
- ThermoPCD: a database of molecular dynamics trajectories of antibody-antigen complexes at physiologic and fever-range temperatures.Database : the journal of biological databases and curation · 2024Article
- Fever as an evolutionary agent to select immune complexes interfaces.Immunogenetics · 2022Article
- Fever temperatures impair hemolysis caused by strains ofHeliyon · 2022Article
- Fever temperatures modulate intraprotein dynamics and enhance the binding affinity between monoclonal antibodies and the spike protein from SARS-CoV-2.Computational and structural biotechnology journal · 2022Article
- Plasmonic Layer as a Localized Temperature Control Element for Surface Plasmonic Resonance-Based Sensors.Sensors (Basel, Switzerland) · 2021Article
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Authors and funding
6 authors.
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Abstract
Fever is a regulated increase of the body temperature resulting from both infectious and non-infectious causes. Fever is known to play a role in modulating immune responses to infection, but the potential of febrile temperatures in regulating antigen binding affinity to antibodies has not been explored. Here we investigated this process under in vitro conditions using Isothermal titration calorimetry and ELISA. We used selected malarial and dengue antigens against specific monoclonal antibodies, and observed a marked increase in the affinity of these antibody-antigen complexes at 40°C, compared to physiological (37°C) or pathophysiological temperatures (42°C). Induced thermal equilibration of the protein partners at these temperatures in vitro, prior to measurements, further increased their binding affinity. These results suggest another positive and adaptive role for fever in vivo, and highlight the favourable role of thermal priming in enhancing protein-protein affinity for samples with limited availability.
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