ArticleMolecular biology of the cell2019
Newly synthesized claudins but not occludin are added to the basal side of the tight junction.
Article in Molecular biology of the cell, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 56 citations in OpenAlex.
- Structure-forward targeting of claudins with synthetic binders.FEBS letters · 2026Review
- Tight junction structure, assembly and (dys)function.Nature reviews. Molecular cell biology · 2026Review
- An epithelial morphogenetic program for maximal urine concentration.Nature communications · 2026Article
- Control of renal calcium permeability via a tight junctional claudin switch.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Biophysical basis of tight junction barrier modulation by a pan-claudin-binding molecule.PNAS nexus · 2025Article
- Regional Heterogeneity in Intestinal Epithelial Barrier Permeability and Mesenteric Perfusion After Thoracic Spinal Cord Injury.Digestive diseases and sciences · 2024Article
- Pumilio-1 mediated translational control of claudin-5 at the blood-brain barrier.Fluids and barriers of the CNS · 2024Article
- Invasive micropapillary carcinoma of the breast and invasive breast carcinoma of no special type: a comparison of claudin proteins' expression and its impact on survival.Pathology oncology research : POR · 2024Article
- Claudin-23 reshapes epithelial tight junction architecture to regulate barrier function.Nature communications · 2023Article
- Storming the gate: New approaches for targeting the dynamic tight junction for improved drug delivery.Advanced drug delivery reviews · 2023Review
- The CLDN5 gene at the blood-brain barrier in health and disease.Fluids and barriers of the CNS · 2023Review
- EpCAM proteolysis and release of complexed claudin-7 repair and maintain the tight junction barrier.The Journal of cell biology · 2023Article
- SNAP- and Halo-tagging and dye introduction protocol for live microscopy inSTAR protocols · 2022Article
- Tight junction channel regulation by interclaudin interference.Nature communications · 2022Article
- Review
- Angulin-1 (LSR) Affects Paracellular Water Transport, However Only in Tight Epithelial Cells.International journal of molecular sciences · 2021Article
- The Epithelial Cell Leak Pathway.International journal of molecular sciences · 2021Review
- Occludin and tricellulin facilitate formation of anastomosing tight-junction strand network to improve barrier function.Molecular biology of the cell · 2021Article
- Localization of Claudin-3 and Claudin-4 within the Small Intestine of newborn piglets.Physiological reports · 2021Article
- TGF-β1 increases permeability of ciliated airway epithelia via redistribution of claudin 3 from tight junction into cell nuclei.Pflugers Archiv : European journal of physiology · 2021Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
A network of claudin strands creates continuous cell-cell contacts to form the intercellular tight junction barrier; a second protein, occludin, is associated along these strands. The physiological barrier remains stable despite protein turnover, which involves removal and replacement of claudins both in the steady state and during junction remodeling. Here we use a pulse-block-pulse labeling protocol with fluorescent ligands to label SNAP/CLIP-tags fused to claudins and occludin to identify their spatial trafficking pathways and kinetics in Madin-Darby canine kidney monolayers. We find that claudins are first delivered to the lateral membrane and, over time, enter the junction strand network from the basal side; this is followed by slow replacement of older claudins in the strands. In contrast, even at early times, newly synthesized occludin is found throughout the network. Taking the results together with our previous documentation of the mechanism for claudin strand assembly in a fibroblast model, we speculate that newly synthesized claudins are added at strand breaks and free ends; these are most common in the basalmost edge of the junction. In contrast, occludin can be added directly within the strand network. We further demonstrate that claudin trafficking and half-life depend on carboxy-terminal sequences and that different claudins compete for tight junction localization.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.