ArticleScientific reports2019
Refinement of a chronic cranial window implant in the rat for longitudinal in vivo two-photon fluorescence microscopy of neurovascular function.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 38 citations in OpenAlex.
- Protein kinase Cδ and pharmacomechanical coupling: Re-envisioning cerebral vascular control.The Journal of physiology · 2026Article
- Corticosterone-linked microglial activity underpins sexually dimorphic neuroplasticity after ketamine anesthesia.Science advances · 2026Article
- Mesoscale Recovery of Microglial and Neuronal Dynamics After Craniotomy Across Wide Cortex in Transgenic Mice.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Functional ultrasound as a quantitative approach for measuring functional hyperemia in aging models.NeuroImage · 2025Article
- Viscocohesive hyaluronan gel enhances stability of intravital multiphoton imaging with subcellular resolution.Neurophotonics · 2025Article
- Optical Devices for the Diagnosis and Management of Spinal Cord Injuries: A Review.Biosensors · 2024Review
- A tough bioadhesive hydrogel supports sutureless sealing of the dural membrane in porcine and ex vivo human tissue.Science translational medicine · 2024Article
- Machine Learning Technology for EEG-Forecast of the Blood-Brain Barrier Leakage and the Activation of the Brain's Drainage System during Isoflurane Anesthesia.Biomolecules · 2023Article
- Advances in cellular resolution microscopy for brain imaging in rats.Neurophotonics · 2023Review
- DNA-AgNC Loaded Liposomes for Measuring Cerebral Blood Flow Using Two-Photon Fluorescence Correlation Spectroscopy.ACS nano · 2023Article
- In vivo methods for imaging blood-brain barrier function and dysfunction.European journal of nuclear medicine and molecular imaging · 2023Review
- Immunohistological responses in mice implanted with Parylene HT - ITO ECoG devices.Frontiers in neuroscience · 2023Article
- EEG biomarkers of activation of the lymphatic drainage system of the brain during sleep and opening of the blood-brain barrier.Computational and structural biotechnology journal · 2023Article
- Cranial window for longitudinal and multimodal imaging of the whole mouse cortex.Neurophotonics · 2022Article
- Cranial and Spinal Window Preparation forExperimental neurobiology · 2022Review
- Cellular-resolution monitoring of ischemic stroke pathologies in the rat cortex.Biomedical optics express · 2021Article
- Role of endothelium-pericyte signaling in capillary blood flow response to neuronal activity.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2021Article
- Cerebrovascular development: mechanisms and experimental approaches.Cellular and molecular life sciences : CMLS · 2021Review
- Through the looking glass: A review of cranial window technology for optical access to the brain.Journal of neuroscience methods · 2021Review
- Cellular correlates of gray matter volume changes in magnetic resonance morphometry identified by two-photon microscopy.Scientific reports · 2021Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Longitudinal studies using two-photon fluorescence microscopy (TPFM) are critical for facilitating cellular scale imaging of brain morphology and function. Studies have been conducted in the mouse due to their relatively higher transparency and long term patency of a chronic cranial window. Increasing availability of transgenic rat models, and the range of established behavioural paradigms, necessitates development of a chronic preparation for the rat. However, surgical craniotomies in the rat present challenges due to craniotomy closure by wound healing and diminished image quality due to inflammation, restricting most rat TPFM experiments to acute preparations. Long-term patency is enabled by employing sterile surgical technique, minimization of trauma with precise tissue handling during surgery, judicious selection of the size and placement of the craniotomy, diligent monitoring of animal physiology and support throughout the surgery, and modification of the home cage for long-term preservation of cranial implants. Immunohistochemical analysis employing the glial fibrillary acidic protein (GFAP) and ionized calcium-binding adaptor molecule-1 (Iba-1) showed activation and recruitment of astrocytes and microglia/macrophages directly inferior to the cranial window at one week after surgery, with more diffuse response in deeper cortical layers at two weeks, and amelioration around four weeks post craniotomy. TPFM was conducted up to 14 weeks post craniotomy, reaching cortical depths of 400 µm to 600 µm at most time-points. The rate of signal decay with increasing depth and maximum cortical depth attained had greater variation between individual rats at a single time-point than within a rat across time.
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