ArticleScientific reports2019
Azoreductase activity of dye-decolorizing bacteria isolated from the human gut microbiota.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 115 citations in OpenAlex.
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- Microbiome-aware oral drug delivery: excipient-microbiome interactions and nanocarrier design rules for predictable formulation performance.Frontiers in pharmacology · 2026Review
- Key findings from 15 years ofApplied and environmental microbiology · 2025Review
- Synthesis and Evaluation of Pseudoglucosinolates Releasing Isothiocyanates in the Presence of Azoreductases.Chembiochem : a European journal of chemical biology · 2025Article
- Diet's impact on gut microbial assemblage in health and disease.The Journal of clinical investigation · 2025Review
- Gut microbiota dysbiosis and associated immune response in systemic lupus erythematosus: impact of disease and treatment.Gut pathogens · 2025Article
- Deciphering the universal role of gut microbiota in pollutant transformation.The ISME journal · 2025Review
- Sequence similarity network analysis of drug- and dye-modifying azoreductase enzymes found in the human gut microbiome.Archives of biochemistry and biophysics · 2024Article
- Gut microbial β-glucuronidases influence endobiotic homeostasis and are modulated by diverse therapeutics.Cell host & microbe · 2024Article
- Biodegradation of commercial textile reactive dye mixtures by industrial effluent adapted bacterial consortium VITPBC6: a potential technique for treating textile effluents.Biodegradation · 2024Article
- The key to intestinal health: a review and perspective on food additives.Frontiers in nutrition · 2024Review
- Isolation, molecular identification, and genomic analysis ofFrontiers in microbiology · 2024Article
- Influence of Different Diets on the Degradation of Sulfasalazine by Colon Bacteria Determined Using MimiColPharmaceuticals (Basel, Switzerland) · 2023Article
- Heterogeneous biocatalytic system for effective decolorization of textile dye effluent.3 Biotech · 2023Review
- Investigating Bio-Inspired Degradation of Toxic Dyes Using Potential Multi-Enzyme Producing Extremophiles.Microorganisms · 2023Review
- The Impact of Food Additives on the Abundance and Composition of Gut Microbiota.Molecules (Basel, Switzerland) · 2023Review
- Toxic effects of Arianor Ebony hair dye on human cells.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2023Article
- Biodegradation, Decolorization, and Detoxification of Di-Azo Dye Direct Red 81 by Halotolerant, Alkali-Thermo-Tolerant Bacterial Mixed Cultures.Microorganisms · 2022Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The gut microbiota enriches the human gene pool and contributes to xenobiotic metabolism. Microbial azoreductases modulate the reduction of azo-bonds, activating produgs and azo polymer-coated dosage forms, or degrading food additives. Here, we aimed to screen the healthy human gut microbiota for food colorant-reducing activity and to characterize factors modulating it. Four representative isolates from screened fecal samples were identified as E. coli (AZO-Ec), E. faecalis (AZO-Ef), E. avium (AZO-Ev) and B. cereus (AZO-Bc). Both AZO-Ef and AZO-Ev decolorized amaranth aerobically and microaerophilically while AZO-Ec and AZO-Bc had higher aerobic reduction rates. The isolates varied in their activities against different dyes, and the azo-reduction activity mostly followed zero-order reaction kinetics, with a few exceptions. Additionally, the isolates had different pH dependence, e.g., AZO-Ec was not affected by pH variation while AZO-Bc exhibited variable degradation kinetics at different pH levels. Cell-free extracts showed NADH-dependent enzymatic activities 14-19 times higher than extracellular fractions. FMN did not affect the reducing activity of AZO-Ef cell-free extract, whereas AZO-Ec, AZO-Ev and AZO-Bc had significantly higher reduction rates in its presence (P values = 0.02, 0.0001 and 0.02, respectively). Using Degenerate primers allowed the amplification of azoreductase genes, whose sequences were 98-99% similar to genes encoding FMN-dependent-NADH azoreductases.
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