ArticleProceedings of the National Academy of Sciences of the United States of America2019
Daisy-chain gene drives for the alteration of local populations.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 105 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
105 citing papers in PubMed.
- Non-invasive ovulation tracking enables genetic engineering in wild rodents.Cell reports methods · 2026Article
- Next-generation insect pest management: genetic innovations and emerging biocontrol strategies.Frontiers in plant science · 2026Review
- Field Trials Need Genetic Localization, Not Geographic Isolation.The American journal of tropical medicine and hygiene · 2025Article
- A Comparative Assessment of Self-limiting Genetic Control Strategies for Population Suppression.Molecular biology and evolution · 2025Article
- Controlling the frequency dynamics of homing gene drives for intermediate outcomes.G3 (Bethesda, Md.) · 2025Article
- Gene drives: an alternative approach to malaria control?Gene therapy · 2025Review
- Improving the suppressive power of homing gene drive by co-targeting a distant-site female fertility gene.Nature communications · 2024Article
- Population suppression with dominant female-lethal alleles is boosted by homing gene drive.BMC biology · 2024Article
- Altering traits and fates of wild populations with Mendelian DNA sequence modifying Allele Sails.Nature communications · 2024Article
- Genes drive organisms and slippery slopes.Pathogens and global health · 2024Review
- Germline Cas9 promoters with improved performance for homing gene drive.Nature communications · 2024Article
- Transgene removal using an in cis programmed homing endonuclease via single-strand annealing in the mosquito Aedes aegypti.Communications biology · 2024Article
- Article
- Modelling daisy quorum drive: A short-term bridge across engineered fitness valleys.PLoS genetics · 2024Article
- A multiplexed, confinable CRISPR/Cas9 gene drive can propagate in caged Aedes aegypti populations.Nature communications · 2024Article
- Transformative Approaches for Sustainable Weed Management: The Power of Gene Drive and CRISPR-Cas9.Genes · 2023Review
- Manipulating the Destiny of Wild Populations Using CRISPR.Annual review of genetics · 2023Review
- Expansions to the MGDrivE suite for simulating the efficacy of novel gene-drive constructs in the control of mosquito-borne diseases.BMC research notes · 2023Article
- The suppressive potential of a gene drive in populations of invasive social wasps is currently limited.Scientific reports · 2023Article
- Evaluating the Mating Competency of Genetically Modified Male Mosquitoes in Laboratory Conditions.Frontiers in tropical diseases · 2023Article
45 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors.
Funding
Abstract
If they are able to spread in wild populations, CRISPR-based gene-drive elements would provide new ways to address ecological problems by altering the traits of wild organisms, but the potential for uncontrolled spread tremendously complicates ethical development and use. Here, we detail a self-exhausting form of CRISPR-based drive system comprising genetic elements arranged in a daisy chain such that each drives the next. "Daisy-drive" systems can locally duplicate any effect achievable by using an equivalent self-propagating drive system, but their capacity to spread is limited by the successive loss of nondriving elements from one end of the chain. Releasing daisy-drive organisms constituting a small fraction of the local wild population can drive a useful genetic element nearly to local fixation for a wide range of fitness parameters without self-propagating spread. We additionally report numerous highly active guide RNA sequences sharing minimal homology that may enable evolutionarily stable daisy drive as well as self-propagating CRISPR-based gene drive. Especially when combined with threshold dependence, daisy drives could simplify decision-making and promote ethical use by enabling local communities to decide whether, when, and how to alter local ecosystems.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.